true The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University. C (3-6 months) Yoh WADA Fluorescent Proteins/lacZ System a3-GFP, Tcirg1<floxGFP> a3-GFP, Tcirg1<floxGFP> <a href='https://brc.riken.jp/mus/pcr04420'>Genotyping protocol -PCR-</a> 開発者 / 機関 : 大阪大学 開発年 : 2008 ES cell : R1 (129X1/SvJ x 129S1/Sv)F1-Kitl<+> C57Bl/6 RBRC04420 開発者 / 機関 : 大阪大学開発年 : 2008ES cell : R1 (129X1/SvJ x 129S1/Sv)F1-Kitl<+>C57Bl/6 FLP/frt system 兄妹交配特記 : ホモで継代可能。ホモは１年未満で繁殖成績低下。繁殖成績が低いその他半年以降、やや健康不良にみえる (毛並みが悪くなる)。その後、水頭症になる個体もある。 R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] Cre/loxP system 兄妹交配 特記 : ホモで継代可能。ホモは１年未満で繁殖成績低下。 繁殖成績が低い その他 半年以降、やや健康不良にみえる (毛並みが悪くなる)。その後、水頭症になる個体もある。 Developed by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University in 2008. This allele contains a single Frt site and a single loxP site in intron 13 and the EGFP coding sequence, followed by another loxP site, inserted into the last exon. B6;129(SJL)-Tcirg1<tm1.1Ywd> B6;129(SJL)-Tcirg1<tm1.1Ywd> 和田　洋 This allele contains a single Frt site and a single loxP site in intron 13 and the EGFP coding sequence, followed by another loxP site, inserted into the last exon. The modified gene encodes a fusion protein in which the C-terminal of TCIRG1 is joined to the N-terminal of EGFP by the linker sequence AALELVDPPGSIAT. EGFP fluorescence and the immunofluorescence signal for the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, are colocalized in the late endocytic compartments of mutant peritoneal macrophages. Cre recombinase excision of the loxP-flanked DNA segment generates a null allele. This allele contains a single Frt site and a single loxP site in intron 13 and the EGFP coding sequence, followed by another loxP site, inserted into the last exon. The modified gene encodes a fusion protein in which the C-terminal of TCIRG1 is joined to the N-terminal of EGFP by the linker sequence AALELVDPPGSIAT. EGFP fluorescence and the immunofluorescence signal for the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, are colocalized in the late endocytic compartments of mutant peritoneal macrophages. Cre recombinase excision of the loxP-flanked DNA segment generates a null allele. C（3〜6か月） 条件を付加する。<br>公表を前提とした学術研究に限る。利用者は事前に寄託者の提供承諾書を得る。本件リソースの使用は共同研究のみに限定する。当該リソースを使用した研究成果の基づき特許等の申請を行う場合は、別途協議を行う。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途、MTA を締結する。 Phage phage P1 loxP sites, Yeast flipase recombination target site, Jellyfish GFP cDNA, Mouse Tcirg1 genomic DNA Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol>