B6;129(FVB)-Tcirg1<tm1.2Ywd> B6;129(FVB)-Tcirg1<tm1.2Ywd> Phage phage P1 loxP site, Mouse Tcirg1 genomic DNA RBRC04421 The gene is truncated after exon 13. It contains a single FRT site and a single loxP site in the remaining exon 13-proximal segment of intron 13. Immunoblot analysis fails to detect the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, in liver lysates of homozygous mutant mice. The gene is truncated after exon 13. It contains a single FRT site and a single loxP site in the remaining exon 13-proximal segment of intron 13. Immunoblot analysis fails to detect the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, in liver lysates of homozygous mutant mice. Yoh WADA <a href='https://brc.riken.jp/mus/pcr04421'>Genotyping protocol -PCR-</a> The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University. 開発者 / 機関 : 大阪大学 開発年 : 2008 ES cell : R1 (129X1/SvJ x 129S1/Sv) F1-Kitl<+> C57Bl/6, FVB 兄妹交配 特記 : ホモで継代不可能。 戻し交配 系統名 (ブリーダー名) : C57Bl/6 (SLC) ホモで不妊／死亡 形態の異常・奇形がある 行動の異常がある 繁殖成績が低い その他 視覚障害。聴覚もおそらく異常。 開発者 / 機関 : 大阪大学開発年 : 2008ES cell : R1 (129X1/SvJ x 129S1/Sv) F1-Kitl<+>C57Bl/6, FVB Developed by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University in 2008. C (3-6 months) a3 mutant, Tcirg1<delta> a3 mutant, Tcirg1<delta> Cre/loxP system The gene is truncated after exon 13. It contains a single loxP site in the remaining exon 13-proximal segment of intron 13. Immunoblot analysis fails to detect the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, in liver lysates of homozygous mutant mice. R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] 条件を付加する。<br>公表を前提とした学術研究に限る。利用者は事前に寄託者の提供承諾書を得る。本件リソースの使用は共同研究のみに限定する。当該リソースを使用した研究成果の基づき特許等の申請を行う場合は、別途協議を行う。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途、MTA を締結する。 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> true 兄妹交配特記 : ホモで継代不可能。戻し交配系統名 (ブリーダー名) : C57Bl/6 (SLC)ホモで不妊／死亡形態の異常・奇形がある行動の異常がある繁殖成績が低いその他視覚障害。聴覚もおそらく異常。 C（3〜6か月） The gene is truncated after exon 13. It contains a single FRT site and a single loxP site in the remaining exon 13-proximal segment of intron 13. Immunoblot analysis fails to detect the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, in liver lysates of homozygous mutant mice. Homozygous mutant mice die within 3-4 weeks after birth. 和田　洋