C (3-6 months)
The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from DEPOSITOR and Prof. Yumiko Saga, National Institute of Genetics. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and /or the DEVELOPER's names as co-authors.
Atsushi SUZUKI
Cre発現細胞で、2xHA-TEV-3xFLAG-nanos2が発現するトランスジェニックマウス。HA抗体とFLAG抗体、TEV proteaseを利用することにより、過剰発現させたnanos2に結合する因子の精製が可能である。
HTF-nanos2 #7
HTF-nanos2 #7
Developed by Dr. Atsushi Suzuki, Interdisciplinary Research Center, Yokohama National University in 2009. B6C3F1受精卵にBACトランスジーンを挿入して作出。ICR(MCH)に戻し交配を行う。
ICR;B6C3-Tg(CAG-cat,-HTF/Nanos2)7
ICR;B6C3-Tg(CAG-cat,-HTF/Nanos2)7
CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), E. coli chloramphenicol acetyltransferase, mouse Nanos2 gene cDNA, human influenza virus Ha (hemagglutinin) tag cDNA, Tobacco Etch Virus (TEV) protease
条件を付加する。<br>利用者は事前に寄託者と国立遺伝学研究所・相賀裕美子教授に連絡をとり、提供承諾を得る。また、寄託後2年間について寄託者に連絡をし、共同研究の要否に関する確認を取り、共同研究が必要な場合には共著とする。
Developed by Dr. Atsushi Suzuki, Interdisciplinary Research Center, Yokohama National University in 2009.B6C3F1受精卵にBACトランスジーンを挿入して作出。ICR(MCH)に戻し交配を行う。
Developed by Dr. Atsushi Suzuki, Interdisciplinary Research Center, Yokohama National University in 2009.
<a href='https://brc.riken.jp/mus/pcr04773'>Genotyping protocol -PCR-</a>
C(3〜6か月)
Carrier x Noncarrier [or Crossing to Jcl:MCH(ICR)]
true
RBRC04773
鈴木 敦
Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>