phage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Epcam genomic DNA Heterozygote X Wild-type [C57BL/6JJcl] Cre/loxP system Hiroshi HAMADA 条件を付加する。<br>研究成果の公表にあたって譲渡者の指定する文献を引用する。Dev. Biol., 371, 136-145 (2012). <a href='https://brc.riken.jp/mus/pcr04799'>Genotyping protocol -PCR-</a> 濱田　博司 Developed by Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. The construct was electroporated into G4 ES cells derived from B6129F1. The SA-IRES-Bgeo was excised by crossing with mice expressing F1p (B6-FLPe transgenic mice). B6;B6129-EpCAM<tm1.1Hmd> B6;B6129-EpCAM<tm1.1Hmd> EpCAM遺伝子のfloxedマウス。エクソン2と3がloxPサイトで挟まれている。 C (3-6 months) true EpCAM floxed EpCAM floxed FLP/frt system Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 371, 136-145 (2012). C（3〜6か月） Exon2 and exon3 of Tacstd1 (Epcam, epithelial cell adhesion molecule) gene flanked by loxp sites. Conditional Tacstd1 deficient mice can be generated by cyossing with tissue-specific Cre mice. Homozygous mice were viable and fertile. RBRC04799 大阪大学大学院生命機能研究科 濱田博司先生。