Fluorescent Proteins/lacZ System CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, Stop, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Favia favus Kikume-Green Red cDNA, chicken poly(A) sequence, mouse Gt(ROSA)26Sor genomic region <a href='https://brc.riken.jp/mus/pcr04847'>Genotyping protocol -PCR-</a> Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Approval form (form D) from the depositor (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/KikGR_JPN.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/KikGR_ENG.docx">English</A>)</li><li>Approval form (form V) from the developer (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/KikGR_form_V.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> 古関 明彦 Developed by Michio Tomura and Osami Kanagawa, Research Center for Allergy and Immunologuy, RIKEN in 2009. true Heterozygote x Wild-type [C57BL/6JJcl] B6-ROSA/kikGR (floxed) KI B6-ROSA/kikGR (floxed) KI RBRC04847 条件を付加する。<br>学術機関の学術研究に限る。利用者は提供承諾書を用いて、事前に寄託者(連絡先:haruhiko.koseki@riken.jp)の承諾を得る。営利機関の利用希望者は事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者はApproval form (form V)を用いて、事前にKikGRの開発者の許可を得る(連絡先:Laboratory for Cell Function Dynamics, RIKEN CBS:mta-cfds@ml.riken.jp)。 pCAGGS expression vector の開発者の大阪大学・宮崎純一博士 (FAX: +81-6-6879-3829、e-mail:jimiyaza@nutri.med.osaka-u.ac.jp) に指定の MTA を提出する。研究成果の公表にあたって謝辞の表明を必要とする。研究成果の公表にあたって寄託者の指定する文献を引用する。Tomura M et.al, Sci. Rep. e6030 (2014). 利用者は本件リソースを用いた研究を公表する際は、事前に寄託者に内容を開示し承諾を得る。利用者は当該発表物1部を寄託者に送付する。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 戻し交配 系統名 (ブリーダー名) : C57BL/6JJcl (日本クレア) C(3〜6か月) 戻し交配系統名 (ブリーダー名) : C57BL/6JJcl (日本クレア) Haruhiko KOSEKI 開発者 / 機関 : 戸村道夫先生・金川修身先生 / 理化学研究所開発年 : 2009年 C (3-6 months) M1 [(C57BL/6 x 129)F1] ROSA locusにCAG-lox-stop-lox-hKikGRをノックインしたマウス。Cre recombinaseにより、photoconvertible fluorescence protein, hKIkGRを発現する。 Cre/loxP system 開発者 / 機関 : 戸村道夫先生・金川修身先生 / 理化学研究所 開発年 : 2009年 The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Lab Contact: haruhiko.koseki@riken.jp) using the Approval Form (form D). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the kikGR using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a MTA regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki. Osaka University (FAX:+81-6-6879-3829, e-mail: jimiyaza@nutri.med.osaka-u.ac.jp). In publishing the research results to be obtained by the use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Tomura M et.al, Sci. Rep. e6030 (2014). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must send the DEPOSITOR a reprint of the RECIPIENT's publication. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. B6.B6129-Gt(ROSA)26Sor<tm1(CAG-kikGR)Kgwa> B6.B6129-Gt(ROSA)26Sor<tm1(CAG-kikGR)Kgwa>