戻し交配 系統名 (ブリーダー名) : C57BL/6J SA (splice acceptor sequence), S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP sites, Yeast FRT (flippase recombination target) site, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), HA (hemagglutinin) tag sequence, Clavularia sp. mTFP1, STOP cassette Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://imayoshi.web.fc2.com/Itaru_Imayoshi_Ph.D./Mouse_strain.html" target="_blank">Itaru Imayoshi HP</A> Cre/loxP system HAtag-attached mTFP1 fluorescent protein is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, HA-mTFP1 is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region. 蛍光たんぱく質mTFP1 (N末にHAタグを付加) が、Cre及びFLPの両方の組み換え後に発現するコンストラクトを、Rosa26遺伝子座にノックインしてある。発現レベルを増強する為に、プロモーターはCAGプロモーターを用いており、またmTFP1の終止コドンとSV40pA配列の間にWPRE配列を挿入してある。キメラマウスをpCAG-FLPeマウスと交配し、FRT-Stop-FRTカセットを除去した。,(HAtag-attached mTFP1 fluorescent protein is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, HA-mTFP1 is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region.) Developed by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. FRT-Stop-FRT cassette was removed by crossing with pCAG-FLPe mice (C57BL/6). FLP/frt system B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo> B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo> TC1 [129S6/SvEvTac] 今吉　格 開発者 / 機関 : 今吉 格 先生・影山 龍一郎先生(Itaru Imayoshi, Ryoichiro Kageyama) / 京都大学ウィルス研究所 (Institute for Virus Research, Kyoto University)開発年 : 2010年 true <a href='https://brc.riken.jp/mus/pcr05147'>Genotyping protocol -PCR-</a> 戻し交配系統名 (ブリーダー名) : C57BL/6J 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Neurosci. Res., 73, 85-91 (2012).<br>学術機関の学術研究に限る。分与希望者は事前に今吉格（E-mail: iimayosh@virus.kyoto-u.ac.jp）に連絡をとり、分与に関して許可を得ること。（提供承諾書（書式6）の提出は不要）。 R26-CAG-LoxP-mTFP1 R26-CAG-LoxP-mTFP1 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 73, 85-91 (2012).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). 開発者 / 機関 : 今吉 格 先生・影山 龍一郎先生(Itaru Imayoshi, Ryoichiro Kageyama) / 京都大学ウィルス研究所 (Institute for Virus Research, Kyoto University) 開発年 : 2010年 Fluorescent Proteins/lacZ System B (1-3 months) B（1〜3か月） RBRC05147 Itaru IMAYOSHI 蛍光たんぱく質mTFP1 (N末にHAタグを付加) が、Cre及びFLPの両方の組み換え後に発現するコンストラクトを、Rosa26遺伝子座にノックインしてある。発現レベルを増強する為に、プロモーターはCAGプロモーターを用いており、またmTFP1の終止コドンとSV40pA配列の間にWPRE配列を挿入してある。キメラマウスをpCAG-FLPeマウスと交配し、FRT-Stop-FRTカセットを除去した。(HAtag-attached mTFP1 fluorescent protein is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, HA-mTFP1 is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region.)