Developed by Shin-Ichi Nishikawa, RIKEN Center for Developmental Biology. Vlk (Acc. No. CDB0434K) Vlk (Acc. No. CDB0434K) Pkdcc, protein kinase domain containing, cytoplasmic knockout mice (24247-venus-LNL). Homozygous mice die soon after birth due to respiratory failure. true B6.Cg-Pkdcc<tm1.1Mask>/Rbrc <a href='https://brc.riken.jp/mus/pcr05275'>Genotyping protocol -PCR-</a> C（3〜6か月） C (3-6 months) Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (<a HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Form_V.docx">Form V (English)</a>) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] 条件を付加する。<br>(1) 当該マウスを用いる論文発表に際してはAcc.No. CDB0434K、そのWEB サイト（http://www2.clst.riken.jp/arg/mutant%20mice%20list.html）とともに当該マウスを記載すること。（2）当該マウスを用いる論文発表に際しては、その由来として第一報論文Development. 2009 136(12):2069-79.を引用すること。（4）発表論文を寄託者（mutant.bdr@riken.jp）に連絡すること。 Developmental Biology Research attB1,Jellyfish venus (GFP) cDNA, phage P1 loxP site, attB2, mouse Pkdcc genomic DNA Heterozygote x Wild-type [C57BL/6N] 理化学研究所 発生・再生科学総合研究センター 西川伸一先生。 Cre/loxP system Vlk (Acc. No. CDB0434K) Fluorescent Proteins/lacZ System RBRC05275 RIKEN CDB RIKEN CDB (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0434K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Development. 2009 136(12):2069-79.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. Vlk遺伝子のノックアウトマウス。Vlk (Pkdcc) はES細胞由来中内胚葉細胞において発現するリン酸化酵素より同定された遺伝子であり、間葉系細胞のストローマ機能に重要な役割を果たす。ホモマウスは生後致死。