E. coli neo, yeast FRT (flipase recombination target) site, phage P1 loxP, mouse phosphoglycerate kinase promoter (PGK promoter), mouse PGK3’end, E. coli lacZ, Rabbit beta globin pA signal, mouse nrd1 genomic DNA C (3-6 months) 条件を付加する。<br>(1) 分与希望者は事前に開発者 (京都大学、西 英一郎博士, E-mail: nishi@kuhp.kyoto-u.ac.jp) に連絡をとり、分与に関して許可を得ること。（2）当該マウスを用いる論文発表に際してはAcc.No. CDB0466K、そのWEB サイト（http://www2.clst.riken.jp/arg/mutant%20mice%20list.html）とともに当該マウスを記載すること。（3）当該マウスを用いる論文発表に際しては、その由来として第一報論文Nat. Neurosci., 2009 12(12):1506-13.を引用すること。（4）発表論文を寄託者（mutant.bdr@riken.jp）に連絡すること。 Heterozygote x Wild-type [C57BL/6N] nrd1 (Acc. No.CDB0466K) nrd1 (Acc. No.CDB0466K) (1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Eiichirou Nishi, E-mail: nishi@kuhp.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0466K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Nat. Neurosci., 2009 12(12):1506-13.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. 京都大学大学院医学系研究科 西 英一郎先生。 Developed by Eiichirou Nishi, Graduate School of Medicine, Kyoto University. true C（3〜6か月） <a href='https://brc.riken.jp/mus/pcr05280'>Genotyping protocol -PCR-</a> Nrd1, nardilysin, N-arginine dibasic convertase, NRD convertase 1 knockout mice (Nrd1 (lacZ)). Homozygous mutant mice mostly die within 48 hours after birth. Surviving mice show small brain and thin cerebral cortex, cortical thinning, hypomyelination, reduced grip strength, impaired coordination and impaired spatial working memory. RBRC05280 RIKEN CDB RIKEN CDB Nrd1遺伝子のノックアウトマウス。神経細胞軸索の成熟と髄鞘形成の制御に重要な遺伝子。ホモ欠損マウスは生後48時間以内にそのほとんどが致死となる。生存したホモマウスは脳が小さく、大脳皮質は薄い。また、末梢神経系で低髄鞘形成を呈す。 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>