理化学研究所 発生・再生科学総合研究センター 近藤　亨先生。 (1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Toru Kondo, RIKEN, E-mail: tkondo@igm.hokudai.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0623K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (PLoS One. 2009 4(8):e6869.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. <a href='https://brc.riken.jp/mus/pcr05284'>Genotyping protocol -PCR-</a> Fluorescent Proteins/lacZ System Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Prominin1 (Acc. No. CDB0623K) B6.Cg-Prom1<tm1Toko>/Rbrc Cre/loxP system FLP/frt system Prominin1遺伝子のノックアウトマウス。Prominin1は神経幹細胞に発現。 Prominin1(floxed-lacZ-DTA), Prominin1 (Acc. No. CDB0623K) Prominin1(floxed-lacZ-DTA), Prominin1 (Acc. No. CDB0623K) phage P1 LoxP, E. coli lacZ, rabbit beta globin pA, diphtheria toxin DT-A, pA, yeast FRT (flipase recombination target) site, mouse Prom1 genomic DNA RBRC05284 条件を付加する。<br>(1) 分与希望者は事前に開発者 (理化学研究所、近藤　亨博士, E-mail: tkondo@igm.hokudai.ac.jp) に連絡をとり、分与に関して許可を得ること。（2）当該マウスを用いる論文発表に際してはAcc.No. CDB0623K、そのWEB サイト（http://www2.clst.riken.jp/arg/mutant%20mice%20list.html）とともに当該マウスを記載すること。（3）当該マウスを用いる論文発表に際しては、その由来として第一報論文PLoS One. 2009 4(8):e6869.を引用すること。（4）発表論文を寄託者（mutant.bdr@riken.jp）に連絡すること。 C (3-6 months) Prom1, prominin 1 knockout mice (Prominin1 (floxed-lacZ-DTA)). Exon 2, which contains the first ATG codon on the Prom1 locus was replaced with floxed-lacZ and DTA. Homozygous mice are viable and fertile. Developed by Toru Kondo, RIKEN Center for Developmental Biology. C（3〜6か月） Heterozygote x Wild-type [C57BL/6N] Prominin1(floxed-lacZ-DTA) Prominin1(floxed-lacZ-DTA) true RIKEN CDB RIKEN CDB TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]