C（3〜6か月） 条件を付加する。<br>1. 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。PLoS One. 2011 6(12):e29141.<br>2. 公開を前提にした学術研究に限る。<br>3. 利用者は事前に、Tg(Aicda-cre)9Mbuに関してはDr. Meinrad Busslinger（Research Institute of Molecular Pathology）に連絡を取り書面で提供契約を締結する。またGt(ROSA)26Sortm1Shoに関してDr. Philippe Soriano（Mount Sinai School of Medicine）に書面で承諾を取る。 This strain has Ighm knockout (Ighm<tm1Cgn>), AID-Tg (Tg(Aicda-cre)9Mbu) and ROSA26 lacZ reporter (Gt(ROSA)26Sor<tm1Sho>) alleles. Immunodeficient. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www2.mfour.med.kyoto-u.ac.jp/en/index.html" target="_blank">Honjo Lab HP</A> Tasuku HONJO herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neomycin phosphotransferase, polyA, mouse Ighm genomic DNA, mouse Aicda genomic DNA (BAC clone RP24-68I7), SV40 nuclear localization sequence, Phage P1 Cre recombinase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human CD2 cDNA, SV40 polyA signal, retrovirus LTR, adenovirus major late transcript splice acceptor (SA) sequence, Phage P1 loxP sites, E.coli lacZ, E. coli neomycin phosphotransferase, bovine growth hormone polyA, mouse ROSA26 locus genomic DNA muMT/Aicda-cre/R26R, B6-Ighm KO/AicdaCre/ROSA26-lacZ muMT/Aicda-cre/R26R, B6-Ighm KO/AicdaCre/ROSA26-lacZ 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. PLoS One. 2011 6(12):e29141.<br>2) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.<br>3) The BIOLOGICAL RESOURCE contains Tg(Aicda-re)9Mbu and Gt(ROSA)26Sortm1Sho. Before executing the MTA between RIKEN BRC and the RECIPIENT, the RECIPIENT shall contact Dr. Meinrad Busslinger, Research Institute of Molecular Pathology and shall execute the material transfer agreement with Research Institute of Molecular Pathology to use Tg(Aicda-cre)9Mbu. The RECIPIENT shall obtain the prior written consent of Dr. Philippe Soriano, Mount Sinai School of Medicine to use Gt(ROSA)26Sortm1Sho. muMT : Klaus Rajewsky博士 (1991年) R26R : Philippe Soriano博士 (1999年) Aicda-cre : Meinrad Busslinger博士 (2008年) R26R [Soriano, P. (1999). Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 21, 70-71.], muMT [Kitamura, D., Roes, J., Kuhn, R., and Rajewsky, K. (1991). A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin mu chain gene. Nature 350, 423-426.], Aicda-cre [Kwon, K., Hutter, C., Sun, Q., Bilic, I., Cobaleda, C., Malin, S., and Busslinger, M. (2008). Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.] Immunity 28, 751-762. を掛け合わせた。 muMT : Klaus Rajewsky博士 (1991年)R26R : Philippe Soriano博士 (1999年)Aicda-cre : Meinrad Busslinger博士 (2008年)R26R [Soriano, P. (1999). Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 21, 70-71.], muMT [Kitamura, D., Roes, J., Kuhn, R., and Rajewsky, K. (1991). A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin mu chain gene. Nature 350, 423-426.], Aicda-cre [Kwon, K., Hutter, C., Sun, Q., Bilic, I., Cobaleda, C., Malin, S., and Busslinger, M. (2008). Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.] Immunity 28, 751-762. を掛け合わせた。 muMT : Klaus Rajewsky, Insitute for Genetics, University of Cologne. R26R : Philippe Soriano, Mount Sinal School of Medicine. Aicda-cre : Meinrad Busslinger. Research Institute of Molecular Pathology. 膜型IgMの遺伝子が破壊されて居り、Bリンパ球を作らない。Aicda遺伝子を含むBACのトランスジェニックであり、AIDの代わりにヒトCD2抗原とCreを発現する。Rosa26遺伝子座にloxP-stop-LoxP構造によりCreによって発現誘導されるLacZレポーターを挿入されており、Acida-creトランスジーンの発現に合わせて細胞がLacZにより不可逆的にラベルされる。 true Cre/loxP system C (3-6 months) 本庶　佑 Ighm : Homozygote x Homozygote ; Tg : Carrier x Carrier ; ROSA26 : Homozygote x Homozygote B6xCBA受精卵にて作製したAicda-cre Tgマウスは少なくとも４世代樹立者によりB6にかけ戻された。そのマウスにB6背景を持つコンジェニックであるR26RとmuMTを順次かけ合わせ、姉妹兄弟交配で維持した。 <a href='https://brc.riken.jp/mus/pcr05550'>Genotyping protocol -PCR-</a> B6.Cg-Ighm<tm1Cgn> Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Sho> B6.Cg-Ighm<tm1Cgn> Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Sho> RBRC05550