Plin5 (Perilipin 5/MLDP/OXPAT/LSDP5) 遺伝子のノックアウトマウス。エクソン2から4をloxPサイトで挟まれたPGK-neoで置換。Plin5は、脂肪滴表面に局在するタンパク質であり、心臓をはじめ、脂肪酸酸化活性の高い組織に高レベルに発現している。Plin5ホモ欠損マウスは、心臓で脂肪滴が観察されず、脂肪酸酸化活性が上昇している。これにより活性酸素種の発生が増大し、生後30週以降では、エコー検査において心機能の低下を示す。 C (3-6 months) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 287, 23852-23863 (2012).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 兵庫県立大学大学院生命理学研究科 山口 智広先生, 大隅 隆先生 (2007)。J1 ES細胞由来。C57BL/6Jマウスへ戻し交配された (N10世代以上)。 B6-Plin5 KO, B6.129S4-Plin5<tm1> B6-Plin5 KO, B6.129S4-Plin5<tm1> Takashi OSUMI Bacteriophage P1 loxP sites, E. coli Neomycin resistance gene, Bovine Growth hormone gene polyadenylation site, Mouse Phosphoglycerate kinase (PGK) gene promoter, mouse perilipin5 genomic DNA Plin5 perilipin 5 gene knockout mice. Exons 2 to 4 were replaced with a floxed PGK-neo cassette. In homozygous mutant mice, heart lipid droplets were absent and the activity of fatty acid oxidation was increased. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Cardiovascular Research Cre/loxP system 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J. Biol. Chem., 287, 23852-23863 (2012).<br>研究成果の公表にあたって謝辞の表明を必要とする。 RBRC05649 J1 [129S4/SvJae] C（3〜6か月） 大隅　隆 B6.129S4-Plin5<tm1Tosu>/Rbrc B6.129S4-Plin5<tm1Tosu>/Rbrc true Developed by Tomohiro Yamaguchi and Takashi Osumi, Graduate School of Life Science, University of Hyogo in 2007. J1 ES cells derived from 129S4/SvJae were used. The mutant mice were backcrossed to C57BL/6JJcl. <a href='https://brc.riken.jp/mus/pcr05649'>Genotyping protocol -PCR-</a>