Katsuaki SATO 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Immunity, 35, 958-971 (2011). <br>非営利機関の利用に限る。利用者は事前に寄託者（佐藤克明 博士）の承諾（英文の「Approval form」）を得る。利用者は別途、「MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes」を提出する。 Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)] Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)] In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 35, 958-971 (2011). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes" specified by the DEPOSITOR and Inserm. B6.Cg-Siglech<tm1.1Ksat> B6.Cg-Siglech<tm1.1Ksat> Cre/loxP system 佐藤　克明 C (3-6 months) 理化学研究所免疫・アレルギー科学総合研究センター・佐藤克明先生 (2009)。B6.Cg-Thy1<a>由来のBruce 4 ES細胞由来。C57BL/6Jマウスに戻し交配 (9世代以上)。 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>MTA for Siglech-IRES2DTREGFP Knock-In Mice for Academic Research Purposes (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/05658_Inserm_MTA.docx ">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol>Depositor's present address:Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki. Fluorescent Proteins/lacZ System true C（3〜6か月） 形質細胞様樹状細胞 (plasmacytoid dendritic cells ; pDCs) の特異的発現分子であるSiglech, sialic acid binding Ig-like lectin (Siglec)-H遺伝子の3’UTRにIRES-DTR (diphtheria toxin receptor)-EGFP cassetteをノックインしたマウス。diphtheria toxin (DT) 非投与ではSiglec-H欠損マウス、DT投与ではpDC特異的欠失マウスとしての利用が可能。pDCsの生体内機能解析に有用。 IRES-DTR-EGFP cassette were inserted in to 3’ untranslated region of Siglech, Siglech sialic acid binding Ig-like lectin H gene. Plasmacytoid dendritic cells (pDCs) from homozygous mutant mice are defect in the transcriptional and cell surface expression of Siglech. In mice with diphtheria toxin (DT) induced specific ablation of pDCs. Developed by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2009. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. The mutant mice were backcross to C57BL/6J. <a href='https://brc.riken.jp/mus/pcr05658'>Genotyping protocol -PCR-</a> RBRC05658 Encephalomyocarditis virus (EMCV) internal ribosomal entry site 2 (ires2), human diphtheria toxin receptor (DTR) corresponds to the heparin binding EGF-like growth factor, Aequorea Victoria EGFP, Phage P1 loxP site, mouse Siglech genomic DNA Bruce 4 [B6.Cg-Thy1<a>] Siglech<dtr>, B6-Siglech/DT-GFP KI Siglech<dtr>, B6-Siglech/DT-GFP KI Immunology and Inflammation Research