大阪大学微生物病研究所 岡部 勝先生 (2005) Testis specific Pmis2 (Myc-tag) gene transgenic mice. Endogenous Pmis2 expression was absent. Tg males are viable. mouse Pmis2 genomic DNA, E. coli Neomycin resistance gene, mouse Calmgin promoter, human Myc tag, mouse Pmis2 cDNA, rabbit polyA signal(以下残存の可能性有)Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A> KO : Homozygote x Homozygote ; Tg : Carrier x Carrier [or Crossing to Jcl:B6D2F1] KO : Homozygote x Homozygote ; Tg : Carrier x Carrier [or Crossing to Jcl:B6D2F1] RBRC05717 Pmis2-Myc Tg Pmis2-Myc Tg D (more than 6 months) 岡部 勝 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell, 23, 2671-2679 (2012).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. Masaru OKABE D(6か月以上) Developmental Biology Research 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Mol. Biol. Cell, 23, 2671-2679 (2012).<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること(https://www.ccb.osaka-u.ac.jp/en/)。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 精巣特異的にPmis2遺伝子 (Myc-tag付) を発現する。内在性Pmis2遺伝子を欠損する。KOマウスの表現型 (雄性不妊) を回復させることが可能な量のPmis2を精巣に発現している。 true Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2005. B6D2-Pmis2<tm1Osb> Tg(Clgn-Spot3Myc)54Osb B6D2-Pmis2<tm1Osb> Tg(Clgn-Spot3Myc)54Osb