B6.Cg-Tg(CAG-tdKaede)15Utr B6.Cg-Tg(CAG-tdKaede)15Utr In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 10871-10876 (2008).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Kaede using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). CAGプロモーター制御下で全身性に蛍光タンパク質Kaedeを発現するトランスジェニックマウス。特に全血球で発現している。全身で緑色蛍光を発するが、紫外 (UV) 光照射赤色蛍光に変化する (photoconversion)。任意の細胞を特異的にマーキングすることができ、経時的なモニターが可能。 Fluorescent Proteins/lacZ System B (1-3 months) Yoshihiro MIWA <a href='https://brc.riken.jp/mus/pcr05737'>Genotyping protocol -PCR-</a> RBRC05737 WK-m15 WK-m15 These transgenic mice expressing a Kaede, photoconvertible fluorescence protein under the control of the CAG promoter. The expression of green fluoresce of Kaede mice can be converted to red on irradiation with UV or violet light. true Developed by Dr. Yoshihiro Miwa, Graduate School of Comprehensive Human Sciences, University of Tsukuba in 2007. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The Kaede cDNA was provided by Dr. Atsushi Miyawaki, RIKEN Brain Science Institute. C57BL/6 background (RBRC05737), albino C57BL/6 background (RBRC05738). CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Trachyphyllia geoffroy Kaede cDNA, SV40 Poly(A) site Carrier x Carrier [Homozygote x Homozygote] Carrier x Carrier [Homozygote x Homozygote] <A HREF="https://mus.brc.riken.jp/ja/mouse_of_month/aug_2016_mm" target="_blank">Mouse of the Month Aug 2016</A><br><A HREF="https://mus.brc.riken.jp/en/mouse_of_month/dec_2020_mm" target="_blank">Mouse of the Month Dec 2020</A> 筑波大学大学院人間総合科学研究科 三輪佳宏先生 (2007)。Kaede遺伝子は理化学研究所脳科学総合研究所 宮脇敦史博士より供与。B6D2F1受精卵へトランスジーンをインジェクションにより作出。C57BL/6コンジェニックライン (RBRC05737) およびアルビノ型C57BL/6コンジェニック (RBRC05738) ラインが育成された。 B（1〜3か月） Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Approval form (form V) from the developer (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Kaede_form_V.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. USA, 105, 10871-10876 (2008).<br>非営利機関の学術研究に限定する。利用者はApproval form (form V)を用いて、事前にKaedeの開発者の許可を得る（連絡先：Laboratory for Cell Function Dynamics, RIKEN CBS:mta-cfds@ml.riken.jp）。 三輪　佳宏