<a href='https://brc.riken.jp/mus/pcr05879'>Genotyping protocol -PCR-</a> Kcnj11 (Kir6.2) 遺伝子のノックアウトマウス。この遺伝子の欠損は膵beta細胞におけるインスリン分泌や神経細胞におけるグルコース感受性に異常をきたす。ホモマウスは膵beta細胞におけるインスリン分泌が障害されるが、末梢組織でのインスリン感受性が亢進しているため軽度の耐糖能低下にとどまる。ホモマウスは生存性には問題ないものの、仔育てがやや不良。ES由来Tyr遺伝子近傍にあるため、ホモマウスはチンチラ色となる。 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci U S A. 1998 95(18):10402-6.The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. C（3〜6か月） Susumu SEINO Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Developed by Dr. Takashi Miki and Susumu Seino, Chiba University Graduate School of Medicine in 1998. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J over 5 generations. Kcnj11 gene knockout mice. An exon of Kcnj11 gene was disrupted by neomycin resistance gene insertion. Homozygous mutant mice are viable and fertile, but show a somewhat low reproductive performance (nurturing) compared to wild-type. mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Kcnj11 genomic DNA Kir6.2 KO, B6-Kcnj11 KO Kir6.2 KO, B6-Kcnj11 KO 千葉大学大学院医学研究院 三木隆司先生、清野 進先生 (1998)。C57BL/6Jマウスに5世代以上戻し交配された。 清野　進 Neurobiology Research RBRC05879 B6;129P2-Kcnj11<tm1Sse> B6;129P2-Kcnj11<tm1Sse> Heterozygote, Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Heterozygote, Homozygote x Homozygote [or Crossing to C57BL/6JJcl] E14 [129P2/OlaHsd] true 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc Natl Acad Sci U S A. 1998 95(18):10402-6.<br>営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。 C (3-6 months)