Vitamin D receptor gene knockout mice. 1.1kb region containing exon 2 of Vdr was replaced with a neomycin resistance cassette. Homozygous mutant mice show vitamin D dependent rickets type II. mouse phosphoglycerate kinase (PGK) genomic DNA, E. coli neo, mouse Vdr genomic DNA Vitamin D receptor遺伝子のノックアウトマウス。ホモマウスはII型クル病を呈する。体は小さく、粗毛、通常飼料では短命、繁殖性は低い。繁殖をさせるためには、高Ca食を給餌する必要がある。骨形成等のVitamin Dに関する研究に有用。ホモ同士の交配による繁殖成績 (高Ca食給餌) : 妊娠率60％、平均産仔数5.7匹、離乳率90％ (4週齢での離乳)。 B6.Cg-Vdr<tm1Ska> B6.Cg-Vdr<tm1Ska> C（3〜6か月） RBRC05885 <a href='https://brc.riken.jp/mus/pcr05885'>Genotyping protocol -PCR-</a> K JISYAGE true Heterozygote x Wild-type B6-Vdr KO B6-Vdr KO Heterozygote x Wild-type C (3-6 months) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Genet., 16, 391-396 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER (Dr. Shigeaki Kato, uskato0525@gmail.com). Developed by Dr. Shigeaki Kato, Institute of Molecular and Cellular Biosciences, University of Tokyo. C57BL/6 and CBA mixed. Developmental Biology Research 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Genet., 16, 391-396 (1997).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>利用者は事前に開発者（加藤茂明博士・uskato0525@gmail.com) の承諾を得る。 Mouse Models for Human Disease 東京大学分子細胞生物学研究所・加藤茂明先生。C57BL/6とCBAの混合背景。 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> 寺社下　浩一 TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]