D (more than 6 months)
Slc5a10 (SGLT5) gene knockout mice. Part of exon 3 through part of 6 was replaced with a PGK-neo cassette. Homozygous mutant mice exhibit abnormal renal reabsorption (increased urinary fructose and mannose excretion). Fatty liver is also observed in mice fed a high fructose diet. Viable and fertile.
C57BL/6N-Slc5a10<tm1Csk>
C57BL/6N-Slc5a10<tm1Csk>
The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR. (Contact info: Mr. Taku Fukuzawa <fukuzawatk@chugai-pharm.co.jp>, Discovery Pharmacology Dept.)
D（6か月以上）
RBRC06160
true
K JISYAGE
Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>
条件を付加する。<br>利用者は寄託者と事前に検体提供覚書を締結すること。（連絡先：創薬薬理研究部 福澤拓 <fukuzawatk@chugai-pharm.co.jp>)
Slc5a10 (SGLT5) 遺伝子のノックアウトマウス。エクソン3から6の一部をPGK-neoカセットで置換。尿中フルクトースおよびマンノースの排泄量が上昇する。フルクトース負荷により脂肪肝を呈する。生存性、繁殖性あり。
SGLT5 knockout mouse, SGLT5 KO, B6-Slc5a10 KO
SGLT5 knockout mouse, SGLT5 KO, B6-Slc5a10 KO
寺社下 浩一
マウス Phosphoglycerate kinase-1遺伝子プロモーター, phage T7 gb2遺伝子プロモーター, 大腸菌 ネオマイシン耐性遺伝子 cDNA, 合成核酸 ポリA付加シグナル
Metabolism Research
Developed by Chugai Pharmaceutical Co., Ltd. ES cells derived from C57BL/6N were used to disrupt the Slc5a10 gene by homologous recombination. C57BL/6N background.
中外製薬株式会社にて開発。C57BL/6Nマウス由来ES細胞を用いて遺伝子ジーンタゲティング法により樹立。C57BL/6N背景。