ETA (Ednra / endothelin receptor type A) 遺伝子のノックアウトマウス。エクソン5と6をneoカセットで置換。ETA mRNAは神経堤由来のectomesenchymal cells of pharyngeal archesならびにcardiac outflow tissues に多く発現しており、branchial arch (鰓弓) と称される部位の発達に重要な役割を果たしている。ホモノックアウトマウスはヒトにおいて重篤なcraniofacial deformitiesとcardiovascular outflow tractに欠陥を有するCATCH22あるいはvelocardiofacial syndromeと呼ばれる疾患に類似のフェノタイプを呈する。エンドセリン (endothelin、ET) は強力な血管収縮作用を有する血管内皮細胞由来のペプチドで、ブタ大動脈の血管内皮細胞培養上清から、強力な血管収縮作用を有する生理活性物質として単離、精製された。多くの哺乳類には、異なる遺伝子によってコードされるET-1、ET-2、ET-3という3種のペプチド異性体が存在する。また、これらのペプチドの前駆体からの最終的な活性物質エンドセリンの産生に必要なエンドセリン変換酵素 (Endothelin Converting Enzyme-1と-2：ECE-1とECE-2) が同定されている。エンドセリンの受容体にはエンドセリンレセプタータイプAとタイプB (ETAとETB)の2種類が存在する。エンドセリンは心血管系に対する作用のみならず、神経堤由来組織の胚発生において重要な役割を演じていることが示唆されている。 E. coli Neomycin resistance gene, mouse RNA polymerase II promoter, bovine growth hormone polyadenylation site, mouse Ednra genomic DNA Heterozygote x Wild-type [C57BL/6J(Crlj)] Heterozygote x Wild-type [C57BL/6J(Crlj)] Masashi YANAGISAWA ETA (Ednra / endothelin receptor type A) gene knockout mice. Exons 5 and 6 were replaced with a neomycin resistant gene. Homozygous mutant mice are perinatal lethal. 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Development, 125, 813-824 (1998).<br>Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. RBRC06162 JH1 [129S7/SvEvBrd] Developmental Biology Research In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 125, 813-824 (1998).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. true ETA/129, B6-Ednra KO ETA/129, B6-Ednra KO 柳沢　正史 Hematological Research Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> B6;129-Ednra<tm1Ywa> B6;129-Ednra<tm1Ywa> Neurobiology Research C（3〜6か月） <a href='https://brc.riken.jp/mus/pcr06162'>Genotyping protocol -PCR-</a> C (3-6 months) Developed by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. JH1 ES cells were used to generate the mutant mice. 129 and C57BL/6 mixed background. The University of Texas Southwestern Medical Center・柳沢正史先生。129とC57BL/6の混合背景。