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    <ns5:addinfo>&lt;a href='https://brc.riken.jp/mus/pcr06216'&gt;Genotyping protocol -PCR-&lt;/a&gt;</ns5:addinfo>
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    <ns5:termsAndConditions xml:lang="ja">条件を付加する。&lt;br&gt;利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。</ns5:termsAndConditions>
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    <skos:altLabel xml:lang="en">MCR Delta FRT/+ (GWAS023), B6-Mc4r floxed</skos:altLabel>
    <skos:altLabel xml:lang="ja">MCR Delta FRT/+ (GWAS023), B6-Mc4r floxed</skos:altLabel>
    <ns5:termsAndConditions xml:lang="en">RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.</ns5:termsAndConditions>
    <dc:description xml:lang="ja">MCR4 (Mc4r) 遺伝子のfloxedマウス。エクソン1がloxPで挟まれている。MCR4は、ヒトBody mass indexの決定因子の候補として同定された遺伝子である。</dc:description>
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    <ns1:colonyMaintenance xml:lang="ja">Heterozygote x Wild-type [C57BL/6JJcl]</ns1:colonyMaintenance>
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    <ns0:brs_depositor xml:lang="en">Haruhiko KOSEKI</ns0:brs_depositor>
    <ns1:strainDevelopment xml:lang="ja">理化学研究所免疫・アレルギー科学総合研究センター・古関明彦先生（2013）。M1 ES細胞由来。ACTB-FLPeマウス（B6）との交配により、FRTで挟まれたneoカセットを除去。</ns1:strainDevelopment>
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    <ns1:donorDNA>phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Mc4r genomic DNA</ns1:donorDNA>
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    <ns5:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: MTA for distribution with RIKEN BRC (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;/ol&gt;</ns5:addinfo>
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    <ns1:expectedDelivery xml:lang="ja">C（3〜6か月）</ns1:expectedDelivery>
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    <dc:description xml:lang="en">MCR4 (Mcr4) gene floxed mice.  Exon 1 was flanked by loxP sites.</dc:description>
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    <dc:description xml:lang="ja">MCR4 (Mc4r) 遺伝子のfloxedマウス。エクソン1がloxPで挟まれている。MCR4は、ヒトBody mass indexの決定因子の候補として同定された遺伝子である。,</dc:description>
    <ns1:expectedDelivery xml:lang="en">C (3-6 months)</ns1:expectedDelivery>
    <ns1:ESCellLine>M1 [(C57BL/6 x 129)F1]</ns1:ESCellLine>
    <ns1:strainDevelopment xml:lang="en">Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology.  M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of floxed mice.  Neo cassette was removed by crossing with ACTB-FLPe mice (C57BL/6 background).  The mutant mice were crossed to C57BL/6J.</ns1:strainDevelopment>
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  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/HealthCertificate_148536">
    <ns0:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC06216"/>
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  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/HealthCertificate_148904">
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  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/HealthCertificate_150239">
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