Susd3遺伝子のノックアウトマウス。13番染色体の49,343,498から49,326,495の17,004bpの領域にZEN-Ub1カセットが挿入されている。ホモマウスは生存性、妊性あり。 古川 貴久 <a href='https://brc.riken.jp/mus/pcr06218'>Genotyping protocol -PCR-</a> Homozygote x Homozygote [or Crossing to C57BL/6NTac] Homozygote x Homozygote [or Crossing to C57BL/6NTac] VGB6 [C57BL/6NTac] 大阪大学蛋白質研究所・大森義裕、古川貴久。Knockout Mouse Project (KOMP) Repositoryより導入したSusd3<tm1(KOMP)Vlcg>を用いて育成。 Cre/loxP system Developed by Yoshihiro Omori and Takahisa Furukawa, Institute for Protein Research, Osaka University. The original allele and mouse were on C57BL/6 background and from the Knockout Mouse Project (KOMP) Repository. Cell Biology Research Furukawa Takahisa RBRC06218 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> C(3〜6か月) The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested. Fluorescent Proteins/lacZ System Velocigene cassette ZEN-Ub1(E. coli Beta-galactosidase, SV40 polyA, phage P1 loxP sites, human ubiquitin C promoter, E. coli neoR gene, mouse PGK polyA), mouse Susd3 genomic DNA true Susd3, sushi domain containing 3 gene knockout mice. A 17004 bp region between 49,343,498-49,326,495 positions of Chromosome 13 was deleted by the insertion of ZEN-Ub1 cassette. Homozygous mutant mice are viable and fertile. 条件を付加する。<br>学術機関の学術研究に限る。利用者は、事前に寄託者の提供承諾(大阪大学とのMTA締結)を得る。利用者は、研究成果の公表にあたって謝辞の表明を必要とともに、本件リソースがKnockout Mouse ProjectおよびKOMP Repositoryを由来としていることを明記すること。 C (3-6 months) Susd3 KO, B6-Susd3 KO Susd3 KO, B6-Susd3 KO C57BL/6-Susd3<tm1(KOMP)Vlcg> C57BL/6-Susd3<tm1(KOMP)Vlcg>