In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>
Heterozygote x Wild-type [or Crossing to C57BL/6JJcl]
Heterozygote x Wild-type [or Crossing to C57BL/6JJcl]
Hmgb1 gene floxed mice. Exons 2 to 4 were flanked by loxP sites. Homozygous mutant mice are viable, but show weak fertility.
Immunology and Inflammation Research
Developed by Hideyuki Yanai and Tadatsugu Taniguchi, Institute of Industrial Science, University of Tokyo and Nobuaki Yoshida, Institute of Medical Science, University of Tokyo in 2011. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6.
C（3〜6か月）
Phage P1 loxP sites, mouse Hmgb1 genomic DNA
谷口 維紹
Hmgb1遺伝子のfloxedマウス。エクソン2から4がloxPで挟まれている。Hmgb1は主に核に局在し、クロマチン構造を安定化し、遺伝子発現の適切な制御に寄与する他、病原体由来核酸と結合して自然免疫応答の惹起、あるいは細胞外へ放出されて障害関連分子パターンとして機能することがしられている。ホモマウスは生存性はあるが、繁殖性は低い。
RBRC06240
Hmgb1 cKO, B6-Hmgb1 floxed
Hmgb1 cKO, B6-Hmgb1 floxed
Tadatsugu TANIGUCHI
B6.129P2-Hmgb1<tm1Ttg>
B6.129P2-Hmgb1<tm1Ttg>
E14.1 [129P2/OlaHsd]
true
東京大学生産技術研究所・柳井秀元先生、谷口維紹先生、東京大学医科学研究所・吉田進昭先生（2011）。E14.1 ES細胞由来。C57BL/6背景。
条件を付加する。<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>学術機関の学術研究に限る。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
<A HREF="https://mus.brc.riken.jp/en/mouse_of_month/jun_2014_mm" target="_blank">Mouse of the Month Jun 2014</A>
C (3-6 months)
<a href='https://brc.riken.jp/mus/pcr06240'>Genotyping protocol -PCR-</a>
Cre/loxP system