EUCOMM (RIKEN BRC) The RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively. <a href='https://brc.riken.jp/mus/pcr06289'>Genotyping protocol -PCR-</a> C57BL/6N-Tyms<tm1a(EUCOMM)Hmgu>/D03 C57BL/6N-Tyms<tm1a(EUCOMM)Hmgu>/D03 C（3〜6か月） C (3-6 months) EUCOMM (IMPC) Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> JM8.N4 [C57BL/6N] En2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, Bacteriophage P1 loxP site, yeast Frt sites, human beta actin promoter, E. coli Neomycin resistance gene RBRC06289 条件を付加する。<br>The RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively. true