SM1[129S6/SvEvTac] Homozygote x Homozygote [or Crossing to C57BL/6J(Crjl)] Homozygote x Homozygote [or Crossing to C57BL/6J(Crjl)] Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> C(3〜6か月) SV40 large T antigen nuclear localization signal (NLS), E. coli lacZ, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Ffar3 genomic DNA B6;129-Ffar3<tm1Reh> B6;129-Ffar3<tm1Reh> Fluorescent Proteins/lacZ System C (3-6 months) Ffar3 (Gpr41) のエクソンをコードする1.2-kb領域をnLacZとfloxed neoカセットで置換。 true 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. U S A. 105, 16767-1672 (2008).<br>Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. Gpr41 KO, B6-Ffar3 KO Cre/loxP system Gpr41 KO, B6-Ffar3 KO 柳沢 正史 The University of Texas Southwestern Medical Center・柳沢正史先生。 <a href='https://brc.riken.jp/mus/pcr06327'>Genotyping protocol -PCR-</a> Ffar3 (Gpr41) のエクソンをコードする1.2-kb領域をnLacZとfloxed neoカセットで置換。, In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A. 105, 16767-1672 (2008).Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. Masashi YANAGISAWA The Gpr41 (Ffar3) exon coding region was replaced with a nLacZ and floxed neo cassette. RBRC06327 Developed by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. SM1 ES cells derived from 129S6/SvEvTac mice were used to generate the mutant mice. The mice were crossed to C57BL/6.