RIKEN BRC <a href='https://brc.riken.jp/mus/pcr06369'>Genotyping protocol -PCR-</a> C (3-6 months) C(3〜6か月) 条件を付加する。<br>The RECIPIENT should acknowledge RIKEN BRC and the International Mouse Phenotyping Consortium for creating and providing of the knockout mice. IMPC The RECIPIENT should acknowledge RIKEN BRC and the International Mouse Phenotyping Consortium for creating and providing of the knockout mice. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: CRISPR/Cas9 genome edited bioresources (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/Broad_MTA_J.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Broad_MTA_E.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> true (All genes were introduced in the form of RNA)Streptococcus pyogenes tracrRNA, crRNA (in the form of sgRNA) and Cas9, Synthetic DNA Flag tag, His tag, SV40 nuclear localization signal (NLS), Simian virus 5 V5 epitope tag, Bovine growth hormone (bGH) polyA signal RBRC06369 C57BL/6N-Fam135b<em1Rbrc> C57BL/6N-Fam135b<em1Rbrc>