Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. E14 [129P2/OlaHsd] Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.bioreg.kyushu-u.ac.jp/saibou/index_en.html" target="_blank">Lab HP</A> true B6;129P2-Fkbp8<tm1Kei> B6;129P2-Fkbp8<tm1Kei> 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>共同研究。 Fkbp38遺伝子のノックアウトマウス。エクソン3から7をPGK-neo (loxP-neo) カセットで置換。FKBP38は、ミトコンドリア依存的アポトーシス抑制作用を有する膜シャペロンタンパク質である。FKBP38は発生期の神経管形成過程において、アポトーシス制御、およびShhシグナル制御機能を示す。FKBP38ノックアウトマウスは出生直後に死亡となり、中枢神経系において二分脊椎を呈し、神経上皮細胞の細胞構築に異常を来す。 Cre/loxP system Developed by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J over 7 times. Keiichi NAKAYAMA C（3〜6か月） Neurobiology Research 九州大学生体防御医学研究所・中山敬一先生。E14 ES細胞を用いて作出。C57BL/6Jへ交配された（N7世代以上）。 Fkbp38 KO, B6-Fkbp38 KO Fkbp38 KO, B6-Fkbp38 KO <a href='https://brc.riken.jp/mus/pcr06397'>Genotyping protocol -PCR-</a> C (3-6 months) Fkbp38 gene knockout mice. Exons 3 to 7 were raplaced with a PGK-neo (loxP-neo) cassette. 中山　敬一 phage P1 loxP sites, mouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, SV40 polyA signal, mouse Fkbp8 genomic DNA RBRC06397 Heterozygote x Wild-type [C57BL/6JJcl] Heterozygote x Wild-type [C57BL/6JJcl]