Heterozygote x Wild-type [C57BL/6J] Heterozygote x Wild-type [C57BL/6J] Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Frem1 genomic DNA Developed by Kiyotoshi Sekiguchi, Institute for Protein Research, Osaka University in 2006. 129 derived ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6. QBRICK KO, B6-Frem1 KO QBRICK KO, B6-Frem1 KO 大阪大学蛋白質研究所・関口清俊先生（2006）。129 ES細胞を用いて作出。 B6;129-Frem1<tm1Ksek> B6;129-Frem1<tm1Ksek> 基底膜分子QBRICK (Frem1) 遺伝子のノックアウトマウス。QBRICK遺伝子の開始ATGコドンがある第2エクソンをネオマイシン耐性遺伝子で置換。ホモノックアウトマウスではQBRICK蛋白質が発現せず、表皮水疱症、腎臓形成不全、合指、潜在眼球症などが不完全な浸透度で認められる。両腎が形成不全の場合は致死となる。 Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 103, 11981-11986 (2006).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. D (more than 6 months) 関口　清俊 D（6か月以上） Developmental Biology Research QBRICK (Frem1) gene knockout mice. Exon 2 was replaced with a neo cassette. Kiyotoshi SEKIGUCHI true 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. USA, 103, 11981-11986 (2006).<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 RBRC06469