Dock5 gene trap mice. The pGTOlxr gene trap vector containing a beta-geo cassette was inserted into the intron 29 of Dock5 gene.
Homozygote x Homozygote
Homozygote x Homozygote
FLP/frt system
pGT0Lxr gene trap cassette (1.5 kb of mouse En2 intron 1, mouse En2 splice acceptor, yeast FRT (flipase recombination target) sites, phage P1 lox71 site, phage P1 loxP site, E.coli lacZ, E.coli neo, SV40 polyA, pUC backbone)
STOCK Dock5<Gt(AN0268)Wtsi>/Shigm
STOCK Dock5<Gt(AN0268)Wtsi>/Shigm
木下 和生
E14TG2aIV [129P2/OlaHsd]
Dock5 KO, Dock5 Gt
Dock5 KO, Dock5 Gt
条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. USA, 105, 15446-15451 (2008).<br>学術機関の学術研究に限る。
<a href='https://brc.riken.jp/mus/pcr06514'>Genotyping protocol -PCR-</a>
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 15446-15451 (2008).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Dock5遺伝子欠損マウス (ジーントラップマウス) 。Beta-geoを含むジーントラップベクター (pGTOlxr) がイントロン29に挿入されている。
Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>
Fluorescent Proteins/lacZ System
Kinoshita, Kazuo
Montreal大学・Jean-Franc Francois Coteらより開発。滋賀県成人病センター・日合 弘先生により維持された。混合背景(129、BLAB/c、他?)。
Cre/loxP system
true
C (3-6 months)
Developed by Jean-Franc Francois Cote, Montreal University. Maintained by Hiroshi Hiai, Shiga Medical Center for Adults. 129, BALB/c and other strain? mixed background.
C(3〜6か月)
RBRC06514