C (3-6 months) 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Endocrinology. 2014 en20141182.<br>研究成果の公表にあたって謝辞の表明を必要とする。 Developed by Keiichi Itoi, Graduate School of Information Sciences, Tohoku University and Kenji Sakimura, Brain Research Institute, Niigata University in 2011. RENKA ES cells were used to generate the mutant mice. RBRC06520 FLP/frt system 東北大学大学院情報科学研究科・井樋慶一先生、新潟大学脳研究所・崎村健司先生（2011）。RENKA ES細胞を用いて作出。 Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Endocrinology. 2014 en20141182.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RENKA [C57BL/6NCrlCrlj] C（3〜6か月） C57BL/6N-Crh<tm2(cre)Ksak> C57BL/6N-Crh<tm2(cre)Ksak> 井樋　慶一 Heterozygote x Wild-type [C57BL/6NJcl] Heterozygote x Wild-type [C57BL/6NJcl] true Crh (CRF, Corticotropin releasing factor) 遺伝子のCreノックインマウス。エクソン2の開始コドンATGにiCre-frt-flanked-PGK-neoカセットが挿入されている。視床下部、分界条床核などのCRF発現細胞にてcre組換え活性を示す。 <a href='https://brc.riken.jp/mus/pcr06520'>Genotyping protocol -PCR-</a> phage P1 improved Cre recombinase DNA, yeast FRT (flipase recombination target) sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter(synthetic), E. coli Neomycin resistance gene, mouse Crh genomic DNA Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> CRF-iCre, B6-Crh/cre KI CRF-iCre, B6-Crh/cre KI Crh (CRF, Corticotropin releasing factor) gene cre knock-in mice. An iCre-frt-flanked PGK-neo cassette was inserted at the translation initiation site of the CRF gene. Itoi, Keiichi Cre/loxP system