Cre/loxP system The Institute of Physical and Chemical Research RBRC06802 C (3-6 months) B6.Cg-Smc2<tm1.1Hiran> B6.Cg-Smc2<tm1.1Hiran> 平野 達也 理化学研究所平野染色体ダイナミクス研究室・西出賢次先生、平野達也先生。iTL BA ES細胞を用いて作出。C57BL/6背景。 FLP/frt system 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。PLoS Genet. , 4;10(12):e1004847. (2014).<br>研究成果の公表にあたって謝辞の表明を必要とする。 iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1] <a href='https://brc.riken.jp/mus/pcr06802'>Genotyping protocol -PCR-</a> Developed by Kenji Nishide and Tatsuya Hirano, Chromosome Dynamics Laboratory, RIKEN. BA1 ES cells (C57BL/6 x 129/SvEv) were targeted by using standard conditional knockout methods by inGenious Targeting Laboratory (NY, USA). The mice were mated with CAG-FLPe transgenic mice to delete the Neo selection cassettes, and subsequently backcrossed to C57BL/6J over eight generations. phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Smc2 genomic DNA Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] Smc2 floxed, B6-Smc2 floxed Smc2 floxed, B6-Smc2 floxed Smc2 gene floxed mice. Condensins I and II are evolutionarily conserved protein complexes and thought to be central to chromosome assembly and segregation among eukaryotes. In Smc2 floxed mice, exons 10-12 of the Smc2 gene (encoding a shared subunit of both condensins) are flanked by loxP sites. Tissue-specific deletion of Smc2 can be generated by crossing with various Cre-expressing mice to study the functions of both condensins. Homozygous floxed mice are viable and fertile. true Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Smc2遺伝子のfloxedマウス。エクソン10-12がloxPで挟まれている。ホモマウスは生存性、妊性あり。 C（3〜6か月） In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet. , 4;10(12):e1004847. (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.