Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://cfds.riken.jp/material/fucci" target="_blank">Lab HP</A> Atsushi MIYAWAKI Tet system Cytomegalovirus CMV enhancer, chicken β-actin promoter, P1 phage loxP sites, E. coli β-galactosidase, E. coli neomycin-resistance gene, Tet transactivator (E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain), SV40 polyA,/ yeast FRT (flipase recombination target) sites (FRT3 and FRT5), chicken β-globin HS4 insulater, E. coli tetO promoter, synthetic polyA, human hGH polyA, mouse PGK polyA, jellyfish CFP/YFP cDNA, Xenopus CaM, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine bGH polyA, E. coli AttB, λphage AttP, mouse PGK promoter, E. coli hygromycin resistance gene STOCK Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>Igs7<tm92.1(tetO-YCX2.60)Hze>/J STOCK Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>Igs7<tm92.1(tetO-YCX2.60)Hze>/J RBRC09365 Creマウスとの交配によりYCX2.60発現マウスが得られる。 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron., 85(5): 942-958 (2015).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR. Fluorescent Proteins/lacZ System C (3-6 months) <a href='https://brc.riken.jp/mus/pcr09365'>Genotyping protocol -PCR-</a> FLP/frt system 2013年Allen Institute for Brain Scienceにて作製。 Ai92(TITL-YCX2.60)(B6;129S-Igs7tm92.1(tetO-YCX2.60)Hze/J)マウスとROSA26-ZtTAマウス(Jackson Lab #012266)を交配。 true 条件を付加する。利用者は事前に寄託者(開発者)の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Neuron., 85(5): 942-958 (2015).<br>学術研究に限る。研究成果の公表にあたって謝辞を表明し、発表内容のコピーを寄託者に送る。 ROSA26-ZtTA;Ai92(TITL-YCX2.60) ROSA26-ZtTA;Ai92(TITL-YCX2.60) C（3〜6か月） Cre/loxP system 2013年Allen Institute for Brain Scienceにて作製。Ai92(TITL-YCX2.60)(B6;129S-Igs7tm92.1(tetO-YCX2.60)Hze/J)マウスとROSA26-ZtTAマウス(Jackson Lab #012266)を交配。 宮脇　敦史