C (3-6 months) Ryr2遺伝子の第一イントロンに,CAG‐Lox‐stop‐lox‐ human KrasG12V‐IRES‐LacZ‐polyAをノックイン A/J-Ryr2<tm1Nobs> 鈴木　昇 Mie University Life Science Research Center A/J-Ryr2<tm1Nobs> A/J-Ryr2<tm1Nobs> 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。 A.129X-Ryr2<tm1(CAG-KRAS<*>G12V,-lacZ)Nobs> Fluorescent Proteins/lacZ System 開発者；鈴木昇先生、2001年 <a href='https://brc.riken.jp/mus/pcr09479'>Genotyping protocol -PCR-</a> CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, yeast HIS3 DNA, Streptomyces alboniger puromycin resistant gene, human K-rasG12V cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 nuclear localizing signal, E. coli lacZ, bovine growth hormone polyadenylation signal, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 poly A additional signal, mouse Ryr2 genomic DNA Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> true RW4 [129X1/SvJ] C（3〜6か月） RBRC09479 Cre/loxP system Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.