ケモカイン受容体XCR1は、ケモカインXCL1の受容体であり、主として樹状細胞の一つのサブセットに高い特異性をもって発現している。本マウスは、XCR1遺伝子座にヒト由来ジフテリア毒素受容体 (DTR (HBEGF) ) と蛍光タンパク質venus (理研BSI宮脇敦史先生由来) との融合タンパク質 (DTRvenus) をコードする遺伝子をノックインしたマウスである。Venusの発現にて、XCR1陽性細胞を同定できると同時に、ジフテリア毒素の投与により一時的に生体においてXCR1陽性細胞を除去できる。,A chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-DTRvenus mice were generated by knocking the gene encoding a fusion protein consisting of human diphteria toxin receptor (DTR or HBEGF) and a fluorescence protein, venus (derived from Dr. Atsushi Miyawaki at BRI) into the murine XCR1 gene locus. In XCR1-DTRvenus mice, XCR1-expressing cells can be detected by venus expression and ablated transiently by the administration of diphteria toxin. ケモカイン受容体XCR1は、ケモカインXCL1の受容体であり、主として樹状細胞の一つのサブセットに高い特異性をもって発現している。本マウスは、XCR1遺伝子座にヒト由来ジフテリア毒素受容体 (DTR (HBEGF) ) と蛍光タンパク質venus (理研BSI宮脇敦史先生由来) との融合タンパク質 (DTRvenus) をコードする遺伝子をノックインしたマウスである。Venusの発現にて、XCR1陽性細胞を同定できると同時に、ジフテリア毒素の投与により一時的に生体においてXCR1陽性細胞を除去できる。A chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-DTRvenus mice were generated by knocking the gene encoding a fusion protein consisting of human diphteria toxin receptor (DTR or HBEGF) and a fluorescence protein, venus (derived from Dr. Atsushi Miyawaki at BRI) into the murine XCR1 gene locus. In XCR1-DTRvenus mice, XCR1-expressing cells can be detected by venus expression and ablated transiently by the administration of diphteria toxin. RBRC09485 Fluorescent Proteins/lacZ System Immunology and Inflammation Research 条件を付加する。<br>1. 利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。　<br>2. 研究成果の公表にあたって寄託者の指定する文献を引用する　J. Immunol., 190, 6071-6082 (2013). <br>3. 学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　<br>4. 利用者はApproval form (form V)を用いて、事前にVenusの開発者の許可を得る。（連絡先：Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp） human HB-EGF (DTR) cDNA, Aequorea coerulescens mutant GFP (venus) cDNA, Bos taurus bGH polyA, Saccharomyces cerevisiae FRT sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Escherichia coli Neomycin resistance gene, HSV TK polyA signal, mouse Xcr1 genomic DNA true 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form (form D). <br>2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.J. Immunol., 190, 6071-6082 (2013). <br>3. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.<br>4. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). 改正　恒康 改正恒康先生（理化学研究所　統合生命医科学研究センター　炎症制御研究チーム　客員主管研究員）が理化学研究所　免疫・アレルギー科学総合研究センターにて2008年頃作製した。C57BL/6 コンジェニック系統。C57BL/6JJclに12世代戻し交配を行った。Y染色体は原系統。Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008. Kaisho, Tsuneyasu XCR1-DTRvenus XCR1-DTRvenus <a href='https://brc.riken.jp/mus/pcr09485'>Genotyping protocol -PCR-</a> Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (<a HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Form_V.docx">Form V (English)</a>) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> A chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-DTRvenus mice were generated by knocking the gene encoding a fusion protein consisting of human diphteria toxin receptor (DTR or HBEGF) and a fluorescence protein, venus (derived from Dr. Atsushi Miyawaki at BRI) into the murine XCR1 gene locus. In XCR1-DTRvenus mice, XCR1-expressing cells can be detected by venus expression and ablated transiently by the administration of diphteria toxin. B (1-3 months) FLP/frt system B6.Cg-Xcr1<tm2(HBEGF/Venus)Ksho> B6.Cg-Xcr1<tm2(HBEGF/Venus)Ksho> B（1〜3か月） Bruce 4 [B6.Cg-Thy1<a>] 改正恒康先生（理化学研究所　統合生命医科学研究センター　炎症制御研究チーム　客員主管研究員）が理化学研究所　免疫・アレルギー科学総合研究センターにて2008年頃作製した。C57BL/6 コンジェニック系統。C57BL/6JJclに12世代戻し交配を行った。Y染色体は原系統。 Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008. <A HREF="https://mus.brc.riken.jp/ja/mouse_of_month/jan_2016_mm" target="_blank">Mouse of the Month Jan 2016</A> Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008.