条件を付加する。<br>In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Regulation of Fas-mediated immune homeostasis by an activation-induced protein, Cyclon. Saint Fleur S, Hoshino A, Kondo K, Egawa T, Fujii H. Blood (2009) 114 (7): 1355-65. The BIOLOGICAL RESOURCE shall be used only for academic research purposes by not-for-profit institutions. Use of the BIOLOGICAL RESOURCE by for-profit institutions or use for commercial research purposes shall require a commercial license from the Depositor. A recipient shall also acquire a prior written consent from the Depositor prior to filing an application for a patent, or intellectual property or other rights based on the results of their use of the BIOLOGICAL RESOURCE. Ccdc86 (coiled-coil domain containing 86, Cyclon) knockout mice. EGFP and pgk-neo cassettes were placed just downstream of translation initiation codon in exon 1 of the gene. Heterozygous mice exhibited reduced Fas expression in activated T lyphocytes. Homozygous mutants are emobryonic lethal. C57BL/6-Ccdc86<tm1Hfuj> C57BL/6-Ccdc86<tm1Hfuj> iTL IC1 [C57BL/6NTac] true P1ファージ　loxP, オワンクラゲ Green Fluorescent Protein (GFP)(緑色蛍光蛋白質), マウス　Cyclon蛋白質遺伝子, マウス phosphoglycerate-kinase 1 (pgk) プロモーター, 大腸菌　ネオマイシン耐性遺伝子 Developed by Hodaka Fujii, New York University in 2009. iTL IC1 ES cell line [C57BL/6NTac] was used. C57BL/6 background. 開発者／機関：藤井　穂高／ニューヨーク大学。開発年：2009年。iTL IC1 ES細胞 [C57BL/6NTac]を使用。C57BL/6背景。 RBRC09601 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Regulation of Fas-mediated immune homeostasis by an activation-induced protein, Cyclon. Saint Fleur S, Hoshino A, Kondo K, Egawa T, Fujii H. Blood (2009) 114 (7): 1355-65. The BIOLOGICAL RESOURCE shall be used only for academic research purposes by not-for-profit institutions. Use of the BIOLOGICAL RESOURCE by for-profit institutions or use for commercial research purposes shall require a commercial license from the Depositor. A recipient shall also acquire a prior written consent from the Depositor prior to filing an application for a patent, or intellectual property or other rights based on the results of their use of the BIOLOGICAL RESOURCE. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> D（6か月以上） Ccdc86 (coiled-coil domain containing 86, 別名Cyclon) 欠損マウス。エキソン１の翻訳開始点下流にEnhanced Green Fluorescent Protein (EGFP) 遺伝子とネオマイシン耐性遺伝子が挿入されている。ヘテロ型は活性化T細胞におけるFas蛋白質の発現が低下している。ホモ型は胎生期／周産期致死 (どの時期かは不明) 。 Cyclon-KO(EN) Cyclon-KO(EN) Cre/loxP system Fluorescent Proteins/lacZ System D (more than 6 months)