開発者：堀 修。開発年：2009年。機関名：金沢大学（大阪大学微生物病研究所との共同開発）。ES細胞：129Sv/J。C57BL6/J 雌と8回戻し交配。Y染色体：C57BL6由来。 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J. Neurochem., 130, 374-387 (2014).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 Ndrg2 (N-myc downstream regulated gene 2) 欠損マウス。Ndrg2遺伝子のexon3-4を欠失、C57BL/6Jに8世代back cross済み。ホモ欠損マウスは繁殖可能。 Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 130, 374-387 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE. Cre/loxP system Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> <a href='https://brc.riken.jp/mus/pcr09668'>Genotyping protocol -PCR-</a> Ndrg2 Knockout mice Ndrg2 Knockout mice Developed by Osamu Hori, Kanazawa University. ES cell line in 129Sv/J background was used. Mice were backcrossed to C57BL/6 for 8 generations. 開発者：堀 修。開発年：2009年。機関名：金沢大学（大阪大学微生物病研究所との共同開発）。ES細胞：129Sv/J。C57BL6/J 雌と8回戻し交配。Y染色体：C57BL6由来。背景系統：C57BL6（C57BL6/J 雌と8回 戻し交配したもの、日本SLCより入手）Y染色体：C57BL6これまでのところ大きな異常はみられない、-/-同士で繁殖可。 RBRC09668 B6.129-Ndrg2<tm1.1Hori> B6.129-Ndrg2<tm1.1Hori> true Ndrg2 knockout mice. Exon 3 and 4 of the Ndrg2 gene were replaced with a pgk-neo cassette and then it was removed by crossing to CAG-Cre mice. C (3-6 months) phage P1 loxP site, mouse Ndrg2 genomic DNA C（3〜6か月） 背景系統：C57BL6（C57BL6/J 雌と8回 戻し交配したもの、日本SLCより入手） Y染色体：C57BL6 これまでのところ大きな異常はみられない、-/-同士で繁殖可。