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    <dc:description xml:lang="ja">ヒストンバリアントHist1h2aaとHist1h2baの両遺伝子を含む3.6 kbの領域をNeoに置き換えたKOマウス。ホモ接合体のオスは不妊、メスは繁殖率が低いと予想されるので、ヘテロ接合体の個体を繁殖に使用。</dc:description>
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    <skos:altLabel xml:lang="en">Th2a- Th2b-</skos:altLabel>
    <skos:altLabel xml:lang="ja">Th2a- Th2b-</skos:altLabel>
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    <ns4:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Approval form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: MTA for distribution with RIKEN BRC (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;/ol&gt;</ns4:addinfo>
    <ns1:ESCellLine>R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl&lt;+&gt;]</ns1:ESCellLine>
    <ns4:application>Cell Biology Research</ns4:application>
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    <ns1:strainDevelopment xml:lang="en">Developed by Shunsuke Fujii, RIKEN in 2014. R1 ES cell line [(129X1/SvJ x 129S1)F1] was used.  After BALB/cA congenic line had been established, this line was backcrossed to C57BL/6J for 5 generations. BALB/cA congenic line is available at RBRC09729.</ns1:strainDevelopment>
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    <rdfs:label xml:lang="en">B6.Cg(C)-Hist1h2aa/Hist1h2ba&lt;tm1Sis&gt;</rdfs:label>
    <rdfs:label>B6.Cg(C)-Hist1h2aa/Hist1h2ba&lt;tm1Sis&gt;</rdfs:label>
    <ns4:addinfo>&lt;a href='https://brc.riken.jp/mus/pcr09728'&gt;Genotyping protocol -PCR-&lt;/a&gt;</ns4:addinfo>
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    <ns1:donorDNA>mouse Phosphoglycerate kinase promoter (PGK promoter), E coli Neomycin resistance gene, mouse Phosphoglycerate kinase polyA signal, mouse Hist1h2aa/Hist1h2ba genomic DNA</ns1:donorDNA>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/25742800"/>
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    <ns1:strainDevelopment xml:lang="ja">理化学研究所・石井俊輔先生（2014年）。R1（(129X1/SvJ x 129S1)F1）ES細胞由来。BALB/cAコンジェニックマウス化（N11）後、C57BL/6J に戻し交配を５回行った。RBRC09729 はBALB/cAコンジェニック系統。</ns1:strainDevelopment>
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    <ns4:termsAndConditions xml:lang="ja">条件を付加する。&lt;br&gt;研究成果の公表にあたって譲渡者の指定する文献を引用する。Cell Stem Cell., 14, 217-227 (2014). Development, 142, 1287-1292 (2015).&lt;br&gt;研究成果の公表にあたって謝辞の表明を必要とする。&lt;br&gt;営利機関の利用希望者は、事前に利用条件等につき理研BRCと合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、理研BRCと別途協議を行う。</ns4:termsAndConditions>
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    <dc:description xml:lang="en">A 3.6 kb region, which covers both Hist1h2aa and Hist1h2ba, was replaced with a neo cassette via homologous recombination. It is expected that male homozygotes are sterile and female homozygotes have low fertility.</dc:description>
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    <ns1:expectedDelivery xml:lang="en">C (3-6 months)</ns1:expectedDelivery>
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    <ns4:termsAndConditions xml:lang="en">In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Stem Cell., 14, 217-227 (2014). Development, 142, 1287-1292 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with RIKEN BRC and must obtain a prior written consent from the RIKEN BRC  RECIPIENT must contact the RIKEN BRC in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.</ns4:termsAndConditions>
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