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    <ns4:addinfo>&lt;a href='https://brc.riken.jp/mus/pcr09762'&gt;Genotyping protocol -PCR-&lt;/a&gt;</ns4:addinfo>
    <dc:description xml:lang="ja">Frmpd1はFREMドメインとPDZドメインを持つ足場蛋白質</dc:description>
    <dcterms:identifier>RBRC09762</dcterms:identifier>
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    <ns4:termsAndConditions xml:lang="ja">条件を付加する。&lt;br&gt;利用者は、事前に寄託者の提供承諾（大阪大学とのMTA締結）を得る。学術機関の学術研究に限る。利用者は提供者との共同研究を行うことを条件とする。</ns4:termsAndConditions>
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    <rdfs:seeAlso rdf:resource="http://www2.brc.riken.jp/lab/animal/detail.php?brc_no=RBRC09762"/>
    <ns1:strainDevelopment xml:lang="en">Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University. B6C3HF1-derived fertilized eggs were used. Mixed genetic background.</ns1:strainDevelopment>
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    <dc:description xml:lang="en">Frmpd1(FERM and PDZ domain containing 1)mutant mice generated by the CRISPR/Cas9. A part of exon 3 was deleted.Deleted sequence: CCCAGGCACTCACG at exon 3</dc:description>
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    <ns4:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: CRISPR/Cas9 genome edited bioresources (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/Broad_MTA_J.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Broad_MTA_E.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;/ol&gt;</ns4:addinfo>
    <ns4:termsAndConditions xml:lang="en">Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).　The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR.</ns4:termsAndConditions>
    <ns6:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_6"/>
    <skos:altLabel xml:lang="ja">Frmpd1 KO2マウス</skos:altLabel>
    <skos:altLabel xml:lang="en">Frmpd1 KO2 mice</skos:altLabel>
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    <ns1:donorDNA>pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]</ns1:donorDNA>
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    <ns6:brs_hasAllele rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/brc_allele2853"/>
    <ns1:isRecombinant rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean"
    >true</ns1:isRecombinant>
    <ns1:expectedDelivery xml:lang="ja">C（3〜6か月）</ns1:expectedDelivery>
    <ns1:expectedDelivery xml:lang="en">C (3-6 months)</ns1:expectedDelivery>
    <ns6:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns6:brs_hasGenomicFeature>
    <ns1:strainDevelopment xml:lang="ja">大阪大学　蛋白質研究所、分子発生学研究室において確立（2016年）。CRISPR/CAS9法によりデリーションを生じた個体を維持。交配歴としては、B6Jと2度掛け合わせ、その後は兄妹交配により維持していた。+/- は雌雄ともに交配可能である。</ns1:strainDevelopment>
    <rdfs:label>B6;B6C3-Frmpd1&lt;em1Tfur&gt;</rdfs:label>
    <rdfs:label xml:lang="en">B6;B6C3-Frmpd1&lt;em1Tfur&gt;</rdfs:label>
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