Developed by Zhang Qian and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. BDF1 background. pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of BC051142 gene CRISPR/Cas9システムにより、精巣に発現するcDNA配列であるBC051142の5塩基を欠損したノックアウトマウス。この欠損はCDS内に生じているため、フレームシフトを誘導する。 開発者/機関:Zhang Qian, 伊川正人/大阪大学開発年:2015兄妹交配により維持していた。ホモ雄の妊孕性の低下は認められない。 B6D2-BC051142<em1Osb> B6D2-BC051142<em1Osb> BC051142 mutant mice generated by the CRISPR/Cas9 technique. BC051142<em1Osb> [Deleted sequence]:5 bp in Exon 4acggtgtgatggtagcagttgtcctgttgatgacatggctcaatgtcttaagggtttttctttcttatttcagAAATAGTTTTGGCTGTCATCCTGACTG[TATTG]GCCCTTGCTATTCTGACTATTCTGATAGCAAGATGGTCACGTCGTAGGAACAATGgtgagtaaccatggcaactcgactccccagaaaagtctcacagggagggaagacaagatgaggcttgagtctgtctctacttacaagataactacagactttgccagagtgctcagagatcttttccacgaggtgactctgct BC051142<-5/-5> BC051142<-5/-5> D (more than 6 months) Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: CRISPR/Cas9 genome edited bioresources (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/Broad_MTA_J.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Broad_MTA_E.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A> The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. D(6か月以上) 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). <br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。研究成果の公表にあたって寄託者の指定する文献を引用する。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 RBRC09791 開発者/機関:Zhang Qian, 伊川正人/大阪大学 開発年:2015 兄妹交配により維持していた。 ホモ雄の妊孕性の低下は認められない。 true