CRISPR/Cas9システムにより、精巣に発現するTktl1遺伝子の11塩基が欠失し、欠失部位に4塩基の挿入が起こった系統。この変異はCDS内で起こり、フレームシフトを誘導する。 Tktl1<delta 11+4/Y> Tktl1<delta 11+4/Y> RBRC09823 開発者／機関：磯谷綾子，伊川正人／大阪大学 開発年：2014 BDF1系統との交配により維持している。 KO雄の妊孕性の低下は認められない。 true pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine betaGH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Tktl1 gene Developed by Ayako Isotani and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. BDF1 background. Tktl1 mutant mice generated by the CRISPR/Cas9 technique. Tktl1<em1Osb> [Deleted sequence], (Inserted sequence): 11 bp deletion and 4 bp insertion at exon 1.GTTCTGCGGCACAACGTAGAGGAAGGATGTCGGAAGCTGAGGCAAGCA[GTGGGATGGCC](GCAT)CACAACGCTGGACCTGATGAGAAGACATTGCAGGTGTTGCGGGACATGGCCAACCGCCTGCGAATCCGTTCCATCAAGGCCACAAATTCCTCGACCACTAGgtaaaaggcctgggaaggccttaccatggggttctttggttgttctttctgaagctgtgaggtgaagggaacctgctgttatcctccattttctgccttctgggtgccacctactgcttctagatgcccatgagtttcattcctcttcagccctggtggcaggggtacacccctgactaattgtgaccaaagttctggaagcctataagacctccttagtaaggaaaaaatctagaacccagtcgggtgaatgagcagtgttccctggtatttatcaagctagctctagtttggcattttacctagaaatggtgttttgtgtataagcaattgcattggaattggggttggtcat Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: CRISPR/Cas9 genome edited bioresources (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/Broad_MTA_J.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Broad_MTA_E.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A> 開発者／機関：磯谷綾子，伊川正人／大阪大学開発年：2014BDF1系統との交配により維持している。KO雄の妊孕性の低下は認められない。 B6D2-Tktl1<em1Osb> D（6か月以上） B6D2-Tktl1<em1Osb> 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). <br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。研究成果の公表にあたって寄託者の指定する文献を引用する。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 D (more than 6 months) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.