Developed by Shinji Sasaki, Shirakawa Institute of Animal Genetics, Japan Livestock Technology Association in 2016. This strain was generated by the CRISPR/CAS9 technique. B6D2 background. B6D2-Anxa10<em1Siag> B6D2-Anxa10<em1Siag> B6D2受精卵の前核にCRISPR/CAS9でAnxa10(NM_001136089)の69-71bpのPAM、72-91bp標的配列として、74-81 bp (Axa10のexon2に位置) の8 bpの欠損したマウス。 RBRC09905 B (1-3 months) Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: CRISPR/Cas9 genome edited bioresources (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/Broad_MTA_J.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Broad_MTA_E.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Anxa10 (annexin A10) mutant mice. 8 bp deletion at exon 2. Anxa10<em1Siag>: 8 bp deletion at exon 2.tttcatggaaattcatacaatctcttaagtgttgatttttttttcccgacagGTCCAAGGAACCATCTTCCCAGCTCCAAATTTCAATCCCATGATGGATGCCCAAATGCTAGGCGGAGCACTCCAAGGATTTGgtaagccttggaaatacattcgttgattatgaaaaaatataacgtaaaagtaaatttctaggcctttaggcccaggcttgagaatgtgacctctatgactcaatgctccaaggt SHINJI SASAKI、2016 true In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. BMC Genomics, 17, 968- (2016). 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。BMC Genomics, 17, 968- (2016). B(1〜3か月) Anxa10 -/- Anxa10 -/- <a href='https://brc.riken.jp/mus/pcr09905'>Genotyping protocol -PCR-</a> pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine betaGH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]