C (3-6 months) RBRC09911 Developed by Hiromasa Funato and Masashi Yanagisawa, University of Tsukuba. C57BL/6N background. true Sik3<Flag> Sik3<Flag> ランダム点突然変異マウスの睡眠異常スクリーニングにより、睡眠時間減少を示すマウス家系Sleepyを樹立した。この表現型は、Sik3遺伝子中のスプライスドナー部位の点突然変異によってもたらされる。Sik3<em3Iiis>は、Sik3がコードするSIK3蛋白のN末側2nd Met直下にFLAG-HAタグをノックインした系統である。We induced random point mutations by ethylnitrosourea (ENU) and screened for dominant sleep and wakefulness abnormalities. Through our screening, we established a mutant pedigree, which we termed Sleepy (Slp), with a markedly prolonged sleep time. Whole-exome sequencing identified a heterozygous single nucleotide substitution at the splice donor site for intron 13 of the Sik3 gene. Further examine this function, we created the mice in which a Flag-tag was inserted into the N terminus of SIK3 (wildtype) using CRISPR/Cas9 technology.C57BL/6N系統由来の野生型受精卵に対し、Sik3（野生型）遺伝子へCRISPR/Cas9システムを用いFLAG-HAタグ配列を挿入したマウス。睡眠覚醒は正常であり、繁殖にも問題がない。 <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/mar_2019_mm" target="_blank">Mouse of the Month Mar 2019</A> C（3〜6か月） We induced random point mutations by ethylnitrosourea (ENU) and screened for dominant sleep and wakefulness abnormalities. Through our screening, we established a mutant pedigree, which we termed Sleepy (Slp), with a markedly prolonged sleep time. Whole-exome sequencing identified a heterozygous single nucleotide substitution at the splice donor site for intron 13 of the Sik3 gene. Further examine this function, we created the mice in which a Flag-tag was inserted into the N terminus of SIK3 (wildtype) using CRISPR/Cas9 technology. ランダム点突然変異マウスの睡眠異常スクリーニングにより、睡眠時間減少を示すマウス家系Sleepyを樹立した。この表現型は、Sik3遺伝子中のスプライスドナー部位の点突然変異によってもたらされる。Sik3<em3Iiis>は、Sik3がコードするSIK3蛋白のN末側2nd Met直下にFLAG-HAタグをノックインした系統である。 We induced random point mutations by ethylnitrosourea (ENU) and screened for dominant sleep and wakefulness abnormalities. Through our screening, we established a mutant pedigree, which we termed Sleepy (Slp), with a markedly prolonged sleep time. Whole-exome sequencing identified a heterozygous single nucleotide substitution at the splice donor site for intron 13 of the Sik3 gene. Further examine this function, we created the mice in which a Flag-tag was inserted into the N terminus of SIK3 (wildtype) using CRISPR/Cas9 technology. C57BL/6N系統由来の野生型受精卵に対し、Sik3（野生型）遺伝子へCRISPR/Cas9システムを用いFLAG-HAタグ配列を挿入したマウス。睡眠覚醒は正常であり、繁殖にも問題がない。 mouse Sik3 genomic DNA, Synthetic FLAG tag, Human Influenza HA tag (Hemagglutinin gene) Mouse line harboring a Flag-tag inserted into the N terminus of SIK3 (wildtype) by the CRISPR/Cas9 technology. This line exhibits normal sleep and wakefulness and breeds well. 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Nature, 539, 378-383 (2016).<br>利用者は提供承諾書を用いて、事前に柳沢正史博士のの承諾を得る。学術機関の学術研究に限る。 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: CRISPR/Cas9 genome edited bioresources (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/Broad_MTA_J.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Broad_MTA_E.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 539, 378-383 (2016).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from　Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. 睡眠覚醒に関わる遺伝子Sik3の野生型アリルへFLAG-HAタグを挿入した系統である。 C57BL/6N-Sik3<em2Iiis> C57BL/6N-Sik3<em2Iiis>