Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol.,134(6), 1701-1709 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Mito-roGFP mice(MROH20) Mito-roGFP mice(MROH20) 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J. Invest. Dermatol.,134(6), 1701-1709 (2014).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>学術機関の学術研究に限る。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 human EF1A promoter, mitchondrial targeting seaquence (human pyruvate dehydrogenase E1 subunit leader sequence, PDHA1), jellyfish GFP(EGFP) cDNA, human G-CSF cDNA PolyA signal true HR1.B6-Hr<hr> Tg(EF1A-PDHL/roGFP1)20Awolf Developed by Alexander M Wolf, G Nippon Medical School in 2012. Transgenic construct was injected into fertilized eggs derived from C57BL/6J. Mice were backcrossed to Hos:HR-1 for more than 10 generations. Mice were maintained as heterozygote. 酸化還元状態により励起波長スペクトルが変化するroGFP蛋白をミトコンドリア局在で発現するマウス。皮膚の酸化還元状態の変化をリアルタイムで測定可能。 Fluorescent Proteins/lacZ System RBRC10163 Transgenic mice expressing ratiometric redox-sensitive GFP (PDHL/roGFP1) in the mitochondria of several tissue under the human EF1A promoter. Redox states can be monitored at skin. C (3-6 months) C57BL/6J系統受精卵を用いて作出(EF1Aプロモーター使用）日本医科大学　Alexnder M Wolf (2012)Hos:HR-1に10世代以上の戻し交配を行う。ヘテロで維持。 C（3〜6か月）