<rdf:RDF
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    <ns0:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/straintype_14"/>
    <ns5:application>FLP/frt system</ns5:application>
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    <ns1:expectedDelivery xml:lang="en">C (3-6 months)</ns1:expectedDelivery>
    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
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    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
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    <ns0:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_6"/>
    <ns1:strainDevelopment xml:lang="en">Deposited by Kenji Sakimura, Niigata University in 2020. Mice were generated using RENKA ES cells derived from C57BL/6N. C57BL/6N genetic background.</ns1:strainDevelopment>
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    <ns6:organism rdf:parseType="Resource">
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    <skos:altLabel xml:lang="ja">Tubb3-Cre</skos:altLabel>
    <skos:altLabel xml:lang="en">Tubb3-Cre</skos:altLabel>
    <ns5:termsAndConditions xml:lang="ja">条件を付加する。&lt;br&gt;研究成果の公表にあたって寄託者の指定する文献を引用する。Cell Rep., 36(4):109427(2021).&lt;br&gt;学術機関の学術研究に限る。</ns5:termsAndConditions>
    <ns1:strainDevelopment xml:lang="ja">新潟大学・崎村建司（2005）RENKA C57BL/6N-derived ES cells (Mishina and Sakimura,2007)を用いて作出。C57BL/6N(チャールスリバー)</ns1:strainDevelopment>
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    <ns1:references rdf:parseType="Resource">
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    <ns5:application>Fluorescent Proteins/lacZ System</ns5:application>
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    <ns5:application>Cre/loxP system</ns5:application>
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    <ns5:resourceTypeLink rdf:resource="http://metadb.riken.jp/terms/xsearch#resource_type_targeting"/>
    <ns5:termsAndConditions xml:lang="en">In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 36(4):109427(2021).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.</ns5:termsAndConditions>
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    <ns1:donorDNA>SV40 large T antigen nuclear localization signal (NLS), Bacteriophage P1 Cre recombinase, EMCV internal ribosomal entry site (IRES), Aequorea victoria EGFP cDNA, Saccharomyces cerevisiae Frt site, mouse Tubb3 genomic DNA</ns1:donorDNA>
    <ns1:ESCellLine>RENKA [C57BL/6NCrlCrlj]</ns1:ESCellLine>
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    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
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    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
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    <dc:description xml:lang="ja">tubulin, beta 3 class III（MGI:107813）の遺伝子座に組換え酵素Cre、IRES配列、蛍光タンパク質EGFPをノックインしたマウス。神経細胞にCreを発現（EGFPの発現は検出感度以下）。FLP/frt組換えによりNeoカセットは除去済み。神経科学研究に有用。</dc:description>
    <rdfs:seeAlso rdf:resource="http://www2.brc.riken.jp/lab/animal/detail.php?brc_no=RBRC11130"/>
    <dc:description xml:lang="en">Cre/IRES/EGFP knockin mice targeted at the Tubb3 gene locus.</dc:description>
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    </ns0:brs_hasGenomicFeature>
    <ns5:addinfo>&lt;a href='https://brc.riken.jp/mus/pcr11130'&gt;Genotyping protocol -PCR-&lt;/a&gt;</ns5:addinfo>
    <ns1:isRecombinant rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean"
    >true</ns1:isRecombinant>
    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns0:brs_hasGenomicFeature>
    <ns1:expectedDelivery xml:lang="ja">C（3〜6か月）</ns1:expectedDelivery>
    <ns0:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_13"/>
    <ns5:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: MTA for distribution with RIKEN BRC (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;GFP Transfer License (&lt;A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude"&gt;English&lt;/A&gt;)&lt;br&gt;Please fill in the &lt;A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc"&gt;Schedule A&lt;/A&gt;, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read &lt;A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"&gt; Schedule B&lt;/A&gt;. &lt;/li&gt;&lt;/ol&gt;</ns5:addinfo>
    <ns0:brs_preservationStatus rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainStatus_3"/>
    <dcterms:identifier>RBRC11130</dcterms:identifier>
    <rdfs:label>C57BL/6N-Tubb3&lt;tm1.1(Cre)Ksak&gt;</rdfs:label>
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  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/HealthCertificate_150499">
    <ns0:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC11130"/>
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  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/HealthCertificate_151087">
    <ns0:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC11130"/>
  </rdf:Description>
  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/HealthCertificate_150819">
    <ns0:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC11130"/>
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