Peptide View

MS/MS Fragmentation of TTCGTPNYVAPEVLSHK
Found in AT4G24400.1, CIPK8 (CBL-INTERACTING PROTEIN KINASE 8); kinase

Match to Query 6658: 1952.869674 from(977.442113,2+)
Title: Elution from: 38.884 to 38.884 scan no 3803 polarity:+
Data file \\MASS-DATA2\share\Orbitrap\2008-04\080402ns\o070510_013TA.mgf

Monoisotopic mass of neutral peptide Mr(calc): 1952.8703
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
T1     : Phospho (ST), with neutral losses 97.9769(shown in table), 0.0000
Ions Score: 29  Expect: 0.014  
Matches (Bold Red): 27/262 fragment ions using 42 most intense peaks
# b b++ b* b*++ b0 b0++ Seq. y y++ y* y*++ y0 y0++ #
1 84.0444 42.5258     66.0338 33.5205 T             17
2 185.0921 93.0497     167.0815 84.0444 T 1772.8636 886.9354 1755.8370 878.4221 1754.8530 877.9301 16
3 345.1227 173.0650     327.1121 164.0597 C 1671.8159 836.4116 1654.7893 827.8983 1653.8053 827.4063 15
4 402.1442 201.5757     384.1336 192.5704 G 1511.7852 756.3963 1494.7587 747.8830 1493.7747 747.3910 14
5 503.1919 252.0996     485.1813 243.0943 T 1454.7638 727.8855 1437.7372 719.3723 1436.7532 718.8802 13
6 600.2446 300.6259     582.2340 291.6207 P 1353.7161 677.3617 1336.6896 668.8484 1335.7055 668.3564 12
7 714.2875 357.6474 697.2610 349.1341 696.2770 348.6421 N 1256.6633 628.8353 1239.6368 620.3220 1238.6528 619.8300 11
8 877.3509 439.1791 860.3243 430.6658 859.3403 430.1738 Y 1142.6204 571.8138 1125.5939 563.3006 1124.6098 562.8086 10
9 976.4193 488.7133 959.3927 480.2000 958.4087 479.7080 V 979.5571 490.2822 962.5305 481.7689 961.5465 481.2769 9
10 1047.4564 524.2318 1030.4298 515.7186 1029.4458 515.2266 A 880.4887 440.7480 863.4621 432.2347 862.4781 431.7427 8
11 1144.5092 572.7582 1127.4826 564.2449 1126.4986 563.7529 P 809.4516 405.2294 792.4250 396.7161 791.4410 396.2241 7
12 1273.5517 637.2795 1256.5252 628.7662 1255.5412 628.2742 E 712.3988 356.7030 695.3723 348.1898 694.3882 347.6978 6
13 1372.6202 686.8137 1355.5936 678.3004 1354.6096 677.8084 V 583.3562 292.1817 566.3297 283.6685 565.3456 283.1765 5
14 1485.7042 743.3557 1468.6777 734.8425 1467.6936 734.3505 L 484.2878 242.6475 467.2613 234.1343 466.2772 233.6423 4
15 1572.7362 786.8718 1555.7097 778.3585 1554.7257 777.8665 S 371.2037 186.1055 354.1772 177.5922 353.1932 177.1002 3
16 1709.7952 855.4012 1692.7686 846.8879 1691.7846 846.3959 H 284.1717 142.5895 267.1452 134.0762     2
17             K 147.1128 74.0600 130.0863 65.5468     1

Error Distribution

NCBI BLAST search of TTCGTPNYVAPEVLSHK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence
28.51952.8703-0.0006TTCGTPNYVAPEVLSHK
26.01952.8703-0.0006TTCGTPNYVAPEVLSHK
14.11952.8703-0.0006TTCGTPNYVAPEVLSHK
5.11952.8743-0.0046VIQFLESPEYNMFTR
3.51952.86790.0018TVTLGDVSDMLTNKRK
3.11952.8718-0.0021IWKFTTMNMWPFQK
2.91952.8743-0.0046IYKLCGSPSEDYWKK
2.91952.86630.0034LDGSTPPEERQTLMQR
2.81952.8743-0.0046VIQFLESPEYNMFTR
2.81952.8728-0.0031DSVIESEEGLKVDSPNR

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