Peptide View

MS/MS Fragmentation of TVQFVDWCPTGFK
Found in AT5G19770.1, TUA3 (tubulin alpha-3)

Match to Query 5509: 1663.705702 from(832.860127,2+)
Title: Elution from: 59.875 to 59.875 scan no 6435 polarity:+
Data file \\MASS-DATA2\share\Orbitrap\2008-04\080402ns\o070510_006ZA.mgf

Monoisotopic mass of neutral peptide Mr(calc): 1663.7105
Fixed modifications: Carbamidomethyl (C)
Variable modifications: 
T10    : Phospho (ST), with neutral losses 0.0000(shown in table), 97.9769
Ions Score: 48  Expect: 0.00012  
Matches (Bold Red): 35/208 fragment ions using 36 most intense peaks
# b b++ b* b*++ b0 b0++ Seq. y y++ y* y*++ y0 y0++ #
1 102.0550 51.5311     84.0444 42.5258 T             13
2 201.1234 101.0653     183.1128 92.0600 V 1563.6701 782.3387 1546.6436 773.8254 1545.6596 773.3334 12
3 329.1819 165.0946 312.1554 156.5813 311.1714 156.0893 Q 1464.6017 732.8045 1447.5752 724.2912 1446.5912 723.7992 11
4 476.2504 238.6288 459.2238 230.1155 458.2398 229.6235 F 1336.5432 668.7752 1319.5166 660.2619 1318.5326 659.7699 10
5 575.3188 288.1630 558.2922 279.6497 557.3082 279.1577 V 1189.4747 595.2410 1172.4482 586.7277 1171.4642 586.2357 9
6 690.3457 345.6765 673.3192 337.1632 672.3351 336.6712 D 1090.4063 545.7068 1073.3798 537.1935 1072.3958 536.7015 8
7 876.4250 438.7161 859.3985 430.2029 858.4145 429.7109 W 975.3794 488.1933 958.3528 479.6801 957.3688 479.1881 7
8 1036.4557 518.7315 1019.4291 510.2182 1018.4451 509.7262 C 789.3001 395.1537 772.2735 386.6404 771.2895 386.1484 6
9 1133.5084 567.2579 1116.4819 558.7446 1115.4979 558.2526 P 629.2694 315.1384 612.2429 306.6251 611.2589 306.1331 5
10 1314.5224 657.7649 1297.4959 649.2516 1296.5119 648.7596 T 532.2167 266.6120 515.1901 258.0987 514.2061 257.6067 4
11 1371.5439 686.2756 1354.5173 677.7623 1353.5333 677.2703 G 351.2027 176.1050 334.1761 167.5917     3
12 1518.6123 759.8098 1501.5858 751.2965 1500.6017 750.8045 F 294.1812 147.5942 277.1547 139.0810     2
13             K 147.1128 74.0600 130.0863 65.5468     1

Error Distribution

NCBI BLAST search of TVQFVDWCPTGFK
(Parameters: blastp, nr protein database, expect=20000, no filter, PAM30)
Other BLAST web gateways

All matches to this query

Score Mr(calc): Delta Sequence
47.91663.7105-0.0048TVQFVDWCPTGFK
0.31663.70250.0032GKLFEDDMNRSTR
0.31663.70250.0032GKLFEDDMNRSTR

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