InterProInterPro Protein Domain record

Naphthoate synthase
http://metadb.riken.jp/db/SciNetS_rib124i/crib124s1rib124u10198i

Naphthoate synthase

InterPro Protein Domain record

description
  • <p>This entry represents an enzyme, naphthoate synthase <db_xref db="EC" dbkey="4.1.3.36 "/>, MenB or dihydroxynaphthoic acid synthetase), which is involved in the fifth step of the menaquinone biosynthesis pathway. Menaquinone (vitamin K2), is an essential quinone used in electron-transfer pathways serving as the major electron carrier during anaerobic growth. In the bacterium, the biosynthetic pathway in bacteria typically comprises 6-7 steps [<cite idref="PUB00013809"/>]. Together with o-succinylbenzoate-CoA ligase (menE <db_xref db="INTERPRO" dbkey="IPR010192"/>),MenB takes 2-succinylbenzoate and converts it into 1,4-di-hydroxy-2-naphthoic acid (DHNA) [<cite idref="PUB00013809"/>, <cite idref="PUB00030125"/>, <cite idref="PUB00033363"/>, <cite idref="PUB00033364"/>]. The conversion of o-succinylbenzoate-CoA ligase to DHNA is mediated by the enzyme DHNA synthase, which is encoded by menB [<cite idref="PUB00033365"/>].</p><p>The protein structure of naphthoate synthase has been expressed in <taxon tax_id="562">Escherichia coli</taxon>, purified and crystallized, and found to have fold characteristics of the crotonase family of enzymes, which is notable for the presence of several highly flexible regions around the active site. The C-terminal region of the protein may play a critical role both in completion of the binding pocket and in stabilisation of the hexamer, suggesting a link between oligomerisation and catalytic activity [<cite idref="PUB00033366"/>].</p><p>Included in this entry are two enzymes from <taxon tax_id="3702">Arabidopsis thaliana</taxon> (Mouse-ear cress) and one from <taxon tax_id="1280">Staphylococcus aureus</taxon>, which are identified as putative enoyl-CoA hydratase/isomerases. These enzymes group with the naphthoate synthases when building a tree and when doing BLAST searches.</p>
label
  • Naphthoate synthase
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