<p>This domain is found at the C-terminal of Tn5-type transposase proteins. Prokaryotic Tn5 transposase makes two types of DNA contacts (cis-contacts for DNA recognition and trans-contacts for catalysis) as well as protein-protein contacts [<cite idref="PUB00034644"/>]. Tn5 transposase employs two cations at its active site during catalysis, which cause conformational changes in the bound DNA and which are required for transposition [<cite idref="PUB00027468"/>]. Tn5 transposase has a RnaseH-type alpha/beta/alpha fold. The C-terminal domain forms an orthogonal alpha-bundle structure.</p><p>More information about these proteins can be found at Protein of the Month: Transposase [<cite idref="PUB00034633"/>].</p>