<p>This entry represents a structural motif found in several DNA repair nucleases, such as Rad1/Mus81/XPF endonucleases (<db_xref db="EC" dbkey="3.1.22"/>) [<cite idref="PUB00026231"/>], and in ATP-dependent helicases. The XPF/Rad1/Mus81-dependent nuclease family specifically cleaves branched structures generated during DNA repair, replication, and recombination, and is essential for maintaining genome stability. The nuclease domain architecture exhibits remarkable similarity to those of restriction endonucleases.</p>