<p>This family describes phosphoribosylglycinamide formyltransferase (GAR transformylase), one of several proteins found in formyltransferase. This enzyme uses formyl tetrahydrofolate as a formyl group donor to produce 5'-phosphoribosyl-N-formylglycinamide. PurT, a different GAR transformylase, uses ATP and formate rather than formyl tetrahydrofolate. Experimental proof includes complementation of <taxon tax_id="562">Escherichia coli</taxon> purN mutants by orthologs from vertebrates (where it is a domain of a multifunctional protein), <taxon tax_id="1423">Bacillus subtilis</taxon>, and <taxon tax_id="3702">Arabidopsis thaliana</taxon>. In phylogenetic analyses, the member from <taxon tax_id="4932">Saccharomyces cerevisiae</taxon> shows a long branchlength but membership in the family, while the formyltetrahydrofolate deformylases form a closely related outgroup.</p>