<p>The binding of SeqA protein to hemimethylated GATC sequences is important in the negative modulation of chromosomal initiation at oriC, and in the formation of SeqA foci necessary for <taxon tax_id="562">Escherichia coli</taxon> chromosome segregation [<cite idref="PUB00008475"/>]. SeqA tetramers are able to aggregate or multimerize in a reversible, concentration-dependent manner [<cite idref="PUB00008475"/>]. Apart from its function in the control of DNA replication, SeqA may also be a specific transcription factor [<cite idref="PUB00008980"/>]. The C-terminal domain binds DNA, binding to fully methylated and hemimethylated GATC sequences at oriC. The structure of the C-terminal domain consists of seven alpha-helices and three-stranded beta-sheet.</p>