開発者／機関：藤原祥高、伊川正人／大阪大学開発年：2015年BDF1×BDF1より得られた受精卵へ4930451I11Rik (FIMP)遺伝子エクソン4の蛋白質コード領域を標的としたCRISPR/Cas9プラスミドをマイクロインジェクションすることで欠損を誘導した。BDF1と数回交配後、ミックスバックグラウンドで兄弟交配により維持している。KO雄マウスは、精子融合不全のため不妊になる。 D (more than 6 months) RBRC10114 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: CRISPR/Cas9 genome edited bioresources (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/Broad_MTA_J.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Broad_MTA_E.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A> Developed by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. 精巣で発現する4930451I11Rik (FIMP)遺伝子のエクソン3および4に246bpの欠損が認められるマウス。 4930451I11Rik<-246/wt>, FIMP 4930451I11Rik<-246/wt>, FIMP 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. U S A,117(17):9393-9400 (2020). doi: 10.1073/pnas.1917060117.<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。研究成果の公表にあたって寄託者の指定する文献を引用する。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A,117(17):9393-9400 (2020). doi: 10.1073/pnas.1917060117.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. true Mutant harboring 246 bp deletion at the 4930451I11Rik gene generated by the genome editing technique. 4930451I11Rik<em2Osb>; 246 bp deletion at Exon 3 and Exon 4GCCTTCTTGCTGTGGCCCGGTTTATTGGAGAGAAGCCTATAACGTTCGTTAAGACAGgtatgaatcagcttgatagaggggtcttcctcctggcggggtctggaaggacgctgttgttcattgtctggcagATTCCAGTCCTGGGCTCTTCCAGAACATCCTGGTGGGGACATTAGTGGTGGCCTTCTTCTTCCTGCTTTTCCAGTTCTGCTTGCATGTgtgagttgtgtctctgtgtcctcgccctaccccttccagaggacccaagtctcctatgtgtccctcctcatctgatctccatactcctcagGAACTTCCAGAAAGGGGCCTAATGAGATATCCAGCCAGAGCCAGCTGAGGTGAGCAGGTGAGCCAGAGACGGAGCCCTGGACCCAGCTGTGAGCCACACACATCTACCCTGCCTTCTGTTGAGAAATAAAGGTGGTGCCTCCTTCCTCCTAGACTGCAGTAGCCCTCTATGTAGCCACCCATCCCCCACCTCCTCCTTCTCCCTGACTTCGAAGTGGCCATTCACACACCCCATTCCTCCCAGGCTGCAGGCG B6D2-4930451I11Rik<em2Osb> D（6か月以上） pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of 4930451I11Rik gene